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Literature summary for 3.2.1.78 extracted from

  • Morrill, J.; Kulcinskaja, E.; Sulewska, A.M.; Lahtinen, S.; Stalbrand, H.; Svensson, B.; Abou Hachem, M.
    The GH5 1,4-beta-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes (2015), BMC Biochem., 16, 26 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Bifidobacterium animalis subsp. lactis

Organism

Organism UniProt Comment Textmining
Bifidobacterium animalis subsp. lactis
-
-
-
Bifidobacterium animalis subsp. lactis Bl-04
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bifidobacterium animalis subsp. lactis

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex G75 gel filtration Bifidobacterium animalis subsp. lactis

Storage Stability

Storage Stability Organism
4°C, 10 mM HEPES, 4 days, no loss of activity at pH 8.0, 75% loss of activity at pH 6.0 Bifidobacterium animalis subsp. lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insoluble mannan + H2O
-
 Bifidobacterium animalis subsp. lactis mannotriose + mannotetraose + mannopentaose
-
 ?
insoluble mannan + H2O
-
 Bifidobacterium animalis subsp. lactis Bl-04 mannotriose + mannotetraose + mannopentaose
-
 ?
konjac glucomannan + H2O highest activity Bifidobacterium animalis subsp. lactis ?
-
 ?
konjac glucomannan + H2O highest activity Bifidobacterium animalis subsp. lactis Bl-04 ?
-
 ?
locust bean galactomannan + H2O
-
 Bifidobacterium animalis subsp. lactis mannotriose + mannotetraose + mannopentaose
-
 ?
locust bean galactomannan + H2O
-
 Bifidobacterium animalis subsp. lactis Bl-04 mannotriose + mannotetraose + mannopentaose
-
 ?
low-viscosity locust bean galactomannan + H2O
-
 Bifidobacterium animalis subsp. lactis ?
-
 ?
mannopentaose + H2O
-
 Bifidobacterium animalis subsp. lactis mannobiose + mannotriose + mannotetraose
-
 ?
mannotetraose + H2O
-
 Bifidobacterium animalis subsp. lactis ?
-
 ?
mannotetraose + H2O
-
 Bifidobacterium animalis subsp. lactis Bl-04 ?
-
 ?
additional information the enzyme displays no activity towards mannotriose, Avicel and guar gum Bifidobacterium animalis subsp. lactis ?
-
 ?
additional information the enzyme displays no activity towards mannotriose, Avicel and guar gum Bifidobacterium animalis subsp. lactis Bl-04 ?
-
 ?

Synonyms

Synonyms Comment Organism
1,4-beta-mannanase
-
 Bifidobacterium animalis subsp. lactis
Man5_8
-
 Bifidobacterium animalis subsp. lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bifidobacterium animalis subsp. lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55.9
-
 unfolding temperature Bifidobacterium animalis subsp. lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
97
-
 Mannopentaose at pH 6.0 and 37°C Bifidobacterium animalis subsp. lactis
1828
-
 low-viscosity locust bean galactomannan at pH 6.0 and 37°C Bifidobacterium animalis subsp. lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bifidobacterium animalis subsp. lactis