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Literature summary for 3.2.1.78 extracted from

  • Song, J.; Kim, S.Y.; Kim, D.H.; Lee, Y.S.; Sim, J.S.; Hahn, B.S.; Lee, C.M.
    Characterization of an inhibitor-resistant endo-1,4-beta-mannanase from the gut microflora metagenome of Hermetia illucens (2018), Biotechnol. Lett., 40, 1377-1387 .
    View publication on PubMed
Search for more literature for 3.2.1.78

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol about 110% activity at 100 mM insect gut metagenome
dithiothreitol about 105% activity at 100 mM insect gut metagenome

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells insect gut metagenome

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile about 55% residual activity at 10% (w/v) insect gut metagenome
Benzene complete inhibition at 10% (w/v) insect gut metagenome
chloroform complete inhibition at 10% (w/v) insect gut metagenome
Co2+ about 90% residual activity at 1 mM insect gut metagenome
CTAB complete inhibition at 10% (v/v) insect gut metagenome
Cyclohexane about 5% residual activity at 10% (w/v) insect gut metagenome
Fe2+ about 35% residual activity at 1 mM insect gut metagenome
Hg2+ complete inhibition at 1 mM insect gut metagenome
Mn2+ about 93% residual activity at 1 mM insect gut metagenome
additional information EDTA has no effect on enzyme activity insect gut metagenome
n-butanol complete inhibition at 10% (w/v) insect gut metagenome
n-Heptanol about 5% residual activity at 10% (w/v) insect gut metagenome
n-hexane about 5% residual activity at 10% (w/v) insect gut metagenome
n-Hexanol about 5% residual activity at 10% (w/v) insect gut metagenome
n-Pentanol about 10% residual activity at 10% (w/v) insect gut metagenome
SDS complete inhibition at 10% (w/v) insect gut metagenome
Toluene about 5% residual activity at 10% (w/v) insect gut metagenome
Triton X-100 about 55% residual activity at 10% (v/v) insect gut metagenome
Tween 20 about 60% residual activity at 10% (v/v) insect gut metagenome
Tween 40 about 58% residual activity at 10% (v/v) insect gut metagenome
Tween 80 about 61% residual activity at 10% (v/v) insect gut metagenome
Urea about 70% residual activity at 100 mM insect gut metagenome

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Mg2+, Ca2+, Ni2+, and Cd2+ insect gut metagenome
Zn2+ about 105% activity at 1 mM insect gut metagenome

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
dimethyl formamide the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
dimethyl sulfoxide the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
Ethanol the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
Methanol the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
propanol the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome

Organism

Organism UniProt Comment Textmining
insect gut metagenome A0A1W6DZL1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 insect gut metagenome

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography insect gut metagenome

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-mannan + H2O
-
 insect gut metagenome ?
-
 ?
guar gum galactomannan + H2O
-
 insect gut metagenome ?
-
 ?
konjac glucomannan + H2O
-
 insect gut metagenome ?
-
 ?
locust bean gum galactomannan + H2O highest activity insect gut metagenome ?
-
 ?
additional information no activity with 4-nitrophenyl beta-D-mannopyranoside insect gut metagenome ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE insect gut metagenome

Synonyms

Synonyms Comment Organism
endo-1,4-beta-mannanase
-
 insect gut metagenome
ManEM17
-
 insect gut metagenome

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 insect gut metagenome

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 more than 60% activity between 45 and 60°C insect gut metagenome

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 below 45°C, the recombinant enzyme remains stable with 95% glycosyl hydrolase activity for 1 h, but quickly loses this function above 55°C insect gut metagenome

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 insect gut metagenome

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 long-term (15 h) pre-exposure to acidic and alkaline conditions (pH 5.0-9.5 at 4°C) does not significantly affect enzymatic function, and at least 65% residual activity is retained insect gut metagenome