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Literature summary for 3.2.1.78 extracted from

  • Ghosh, A.; Verma, A.K.; Tingirikari, J.R.; Shukla, R.; Goyal, A.
    Recovery and purification of oligosaccharides from copra meal by recombinant endo-beta-mannanase and deciphering molecular mechanism involved and its role as potent therapeutic agent (2015), Mol. Biotechnol., 57, 111-127.
    View publication on PubMed
Search for more literature for 3.2.1.78

Application

Application Comment Organism
medicine mixed maltooligosacchrides produced by the enzyme exhibit in vitro anti-tumorigenic activity on human colon cancer HT29 cells Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus ATCC 27405
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information production of mannooligosaccharides from pretreated and defatted copra meal hydrolysis by the enzyme Acetivibrio thermocellus ?
-
 ?
additional information production of mannooligosaccharides from pretreated and defatted copra meal hydrolysis by the enzyme Acetivibrio thermocellus ATCC 27405 ?
-
 ?

Subunits

Subunits Comment Organism
More the three-dimensional enzyme structure contains a (beta/alpha)8 TIM barrel folding motif Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
CtManf
-
 Acetivibrio thermocellus
endo-beta-(1->4)-mannanase
-
 Acetivibrio thermocellus
endo-beta-mannanase
-
 Acetivibrio thermocellus
endo-mannanase
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 assay at Acetivibrio thermocellus

General Information

General Information Comment Organism
additional information catalytic residues are Glu181 as catalytic acid/base and Glu288 as nucleophile, molecular docking study with different manno-configured ligands from mannobiose to mannohexose as well as galactomannan. The ability to accommodate larger ligand molecules in the active site of CtManT is probably due to the long loops enclosing the active site that provides the depth of the cavity, structure overview Acetivibrio thermocellus