Any feedback?
Literature summary for 3.2.1.78 extracted from
- Molecular cloning and biochemical characterization of an endo-beta-mannanase gene from soybean for soybean meal improvement (2011), J. Agric. Food Chem., 59, 4622-4628.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM, 50% residual activity | Glycine max |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
locust bean gum | KM value is 34.9 mg/ml, pH 5.0, 28°C | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Glycine max | enzyme is active in hydrolyzing the beta-mannan-rich cell wall of soybean seeds | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | specifically expressed in cotyledons of seedling | Glycine max | - |
| seedling | specifically expressed in cotyledons of seedling | Glycine max | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Glycine max | ? | - |
? | |
| additional information | enzyme is active in hydrolyzing the beta-mannan-rich cell wall of soybean seeds | Glycine max | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Man1 | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable up to | Glycine max |
| 50 | - |
30 min, 20% residual activity | Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.2 | - |
locust bean gum | pH 5.0, 28°C | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Glycine max |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
