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Literature summary for 3.2.1.78 extracted from
- An endo-beta-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai (2010), Comp. Biochem. Physiol. B, 157, 137-143.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 153% of initial activity | Aplysia kurodai |  |
| dithiothreitol | 129% of initial activity | Aplysia kurodai | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 48% residual activity | Aplysia kurodai | |
| Fe3+ | 30.6% residual activity | Aplysia kurodai | |
| Mg2+ | 84% residual activity | Aplysia kurodai | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Aplysia kurodai |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aplysia kurodai | - |
member of glycosyl hydrolase family 5 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive juice | - |
Aplysia kurodai | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.4 | - |
pH 7.0, 30°C | Aplysia kurodai |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum galactomannan + H2O | - |
Aplysia kurodai | ? | - |
? | |
| konjac glucomannan + H2O | - |
Aplysia kurodai | ? | - |
? | |
| additional information | preferably degrades a linear beta-1,4-mannan from green algae Codium fragile producing tri- and disaccharides. no substrate: carboxymethyl cellulose, agarose, dextran and xylan | Aplysia kurodai | ? | - |
? | |
| tara gum galactomannan + H2O | - |
Aplysia kurodai | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Aplysia kurodai |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aplysia kurodai |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
20 min, 50% residual activity | Aplysia kurodai |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aplysia kurodai |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | - |
Aplysia kurodai |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
40°C, 20 min, stable | Aplysia kurodai |
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Release 2023.1 (January 2023)
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