Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 236% of initial activity | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 35% of initial activity | Aspergillus niger | |
Sodium dodecyl sulfate | 20% of initial activity | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for konjac glucomannan of low viscosity, locust bean gum galactomannan, carob galactomannan of low viscosity, and 1,4-beta-D-mannan from carob are 0.6 mg/ml, 2.0 mg/ml, 2.2 mg/ml and 1.5 mg/ml, respectively | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | recombinant enzyme | Aspergillus niger | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 110% of initial activity | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | B6V876 | stran BK01 | - |
Aspergillus niger BK01 | B6V876 | stran BK01 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus niger |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-beta-D-mannan + H2O | - |
Aspergillus niger | ? | - |
? | |
1,4-beta-D-mannan + H2O | - |
Aspergillus niger BK01 | ? | - |
? | |
birch wood xylan + H2O | 9.1% of the activity with locust bean gum | Aspergillus niger | ? | - |
? | |
birch wood xylan + H2O | 9.1% of the activity with locust bean gum | Aspergillus niger BK01 | ? | - |
? | |
carob galactomannan + H2O | - |
Aspergillus niger | ? | - |
? | |
glucomannan + H2O | - |
Aspergillus niger | ? | - |
? | |
glucomannan + H2O | - |
Aspergillus niger BK01 | ? | - |
? | |
guar gum + H2O | 18% of the activity with locust bean gum | Aspergillus niger | ? | - |
? | |
guar gum + H2O | 18% of the activity with locust bean gum | Aspergillus niger BK01 | ? | - |
? | |
locust bean gum galactomannan + H2O | - |
Aspergillus niger | mannobiose + glucosyl-mannosyl oligosaccharides | main product, with low amounts of mannotriose and higher manno-oligosaccharides | ? | |
additional information | negligible activity with starch, carboxymethyl cellulose, and alpha-cellulose | Aspergillus niger | ? | - |
? | |
additional information | negligible activity with starch, carboxymethyl cellulose, and alpha-cellulose | Aspergillus niger BK01 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life 56 h, pH 4.0 | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
148 | - |
1,4-beta-D-Mannan | 70°C | Aspergillus niger | |
215 | - |
glucomannan | 70°C | Aspergillus niger | |
292 | - |
carob galactomannan | 70°C | Aspergillus niger | |
330 | - |
locust bean gum galactomannan | 70°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Aspergillus niger |