Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, 11% increase in activity | Haliotis discus hannai | |
Ca2+ | 1 mM, 20% increase in activity | Haliotis discus hannai |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, complete loss of activity | Haliotis discus hannai | |
Co2+ | 1 mM, 50% loss of activity | Haliotis discus hannai | |
Cu2+ | 1 mM, 49% loss of activity | Haliotis discus hannai | |
dithiothreitol | 1 mM, 25% loss of activity | Haliotis discus hannai | |
Fe2+ | 1 mM, 40% loss of activity | Haliotis discus hannai |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE, x * 39627, calculated for mature protein | Haliotis discus hannai |
39627 | - |
x * 39000, SDS-PAGE, x * 39627, calculated for mature protein | Haliotis discus hannai |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haliotis discus hannai | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a signal peptide of 17 amino acids | Haliotis discus hannai |
Purification (Comment) | Organism |
---|---|
from digestive fluid | Haliotis discus hannai |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive juice | - |
Haliotis discus hannai | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
pH 7.0, 30°C, substrate beta-1,4-mannan | Haliotis discus hannai |
9.6 | - |
pH 7.0, 30°C, substrate glucomannan | Haliotis discus hannai |
11.48 | - |
pH 7.0, 30°C, substrate galactomannan | Haliotis discus hannai |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-mannan + H2O | - |
Haliotis discus hannai | beta-1,4-mannotriose + beta-1,4-mannotetraose | - |
? | |
galactomannan + H2O | source of substrate locust bean gum | Haliotis discus hannai | galactosyl-mannosyl oligosaccharides | - |
? | |
glucomannan + H2O | source of substrate konjac | Haliotis discus hannai | glucosyl-mannosyl oligosaccharides | - |
? | |
additional information | enzyme degrades either beta-1,4-mannan or beta-1,4-mannooligosaccharides to mannotriose and mannotetraose. Enzyme disperses the fronds of the red alga Porphyra yezoensis into cell masses consisting of 10-20 cells that are available for cell engineering | Haliotis discus hannai | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE, x * 39627, calculated for mature protein | Haliotis discus hannai |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Haliotis discus hannai |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, stable | Haliotis discus hannai |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Haliotis discus hannai |