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Literature summary for 3.2.1.78 extracted from

  • Ootsuka, S.; Saga, N.; Suzuki, K.; Inoue, A.; Ojima, T.
    Isolation and cloning of an endo-beta-1,4-mannanase from Pacific abalone Haliotis discus hannai (2006), J. Biotechnol., 125, 269-280.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, 11% increase in activity Haliotis discus hannai
Ca2+ 1 mM, 20% increase in activity Haliotis discus hannai

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete loss of activity Haliotis discus hannai
Co2+ 1 mM, 50% loss of activity Haliotis discus hannai
Cu2+ 1 mM, 49% loss of activity Haliotis discus hannai
dithiothreitol 1 mM, 25% loss of activity Haliotis discus hannai
Fe2+ 1 mM, 40% loss of activity Haliotis discus hannai

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, SDS-PAGE, x * 39627, calculated for mature protein Haliotis discus hannai
39627
-
x * 39000, SDS-PAGE, x * 39627, calculated for mature protein Haliotis discus hannai

Organism

Organism UniProt Comment Textmining
Haliotis discus hannai
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide of 17 amino acids Haliotis discus hannai

Purification (Commentary)

Purification (Comment) Organism
from digestive fluid Haliotis discus hannai

Source Tissue

Source Tissue Comment Organism Textmining
digestive juice
-
Haliotis discus hannai
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
pH 7.0, 30°C, substrate beta-1,4-mannan Haliotis discus hannai
9.6
-
pH 7.0, 30°C, substrate glucomannan Haliotis discus hannai
11.48
-
pH 7.0, 30°C, substrate galactomannan Haliotis discus hannai

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-mannan + H2O
-
Haliotis discus hannai beta-1,4-mannotriose + beta-1,4-mannotetraose
-
?
galactomannan + H2O source of substrate locust bean gum Haliotis discus hannai galactosyl-mannosyl oligosaccharides
-
?
glucomannan + H2O source of substrate konjac Haliotis discus hannai glucosyl-mannosyl oligosaccharides
-
?
additional information enzyme degrades either beta-1,4-mannan or beta-1,4-mannooligosaccharides to mannotriose and mannotetraose. Enzyme disperses the fronds of the red alga Porphyra yezoensis into cell masses consisting of 10-20 cells that are available for cell engineering Haliotis discus hannai ?
-
?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE, x * 39627, calculated for mature protein Haliotis discus hannai

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Haliotis discus hannai

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
30 min, stable Haliotis discus hannai

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Haliotis discus hannai