Crystallization (Comment) | Organism |
---|---|
- |
Cellvibrio japonicus |
Protein Variants | Comment | Organism |
---|---|---|
D283A | increase of Km-value by about 80% | Cellvibrio japonicus |
D283H | dramatic decrease of Km-value | Cellvibrio japonicus |
E212A | dramatic decrease of Km-value | Cellvibrio japonicus |
H143A | decrease of Km-value by about 50% | Cellvibrio japonicus |
H211A | decrease of Km-value by about 30% | Cellvibrio japonicus |
H211N | dramatic decrease of Km-value | Cellvibrio japonicus |
H211N/D283H | decrease of Km-value by about 50% | Cellvibrio japonicus |
W156A | increase of Km-valueby almost 80% | Cellvibrio japonicus |
W162A | increase of Km-value by about 50% | Cellvibrio japonicus |
W217A | increase of Km-value by more than 100% | Cellvibrio japonicus |
Y285A | dramatic decrease of Km-value | Cellvibrio japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00066 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant E212A | Cellvibrio japonicus | |
0.00074 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant Y285A | Cellvibrio japonicus | |
0.0014 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant D283H | Cellvibrio japonicus | |
0.0037 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant H211A/D283A | Cellvibrio japonicus | |
0.0068 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant H211A | Cellvibrio japonicus | |
0.0093 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, wild type | Cellvibrio japonicus | |
0.01 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant H211N | Cellvibrio japonicus | |
0.0141 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant W162A | Cellvibrio japonicus | |
0.0149 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant W156A | Cellvibrio japonicus | |
0.0166 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant D283A | Cellvibrio japonicus | |
0.0205 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant W217A | Cellvibrio japonicus | |
0.04 | - |
2,4-dinitrophenyl-beta-mannobioside | pH 6.5, mutant H143A | Cellvibrio japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellvibrio japonicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl-beta-mannobioside + H2O | - |
Cellvibrio japonicus | 2,4-dinitrophenol + beta-mannobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Trp360 is critical in substrate binding at -1 subsite, Tyr285 works as nucleophile catalyst, Trp217 and Trp162 are important for the activity against mannooligosaccharides but less important for activity against polysaccharides | Cellvibrio japonicus |