Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.78 extracted from

  • Hogg, D.; Woo, E.J.; Bolam, D.N.; McKie, V.A.; Gilbert, H.J.; Pickersgill, R.W.
    Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding (2001), J. Biol. Chem., 276, 31186-31192.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Cellvibrio japonicus

Protein Variants

Protein Variants Comment Organism
D283A increase of Km-value by about 80% Cellvibrio japonicus
D283H dramatic decrease of Km-value Cellvibrio japonicus
E212A dramatic decrease of Km-value Cellvibrio japonicus
H143A decrease of Km-value by about 50% Cellvibrio japonicus
H211A decrease of Km-value by about 30% Cellvibrio japonicus
H211N dramatic decrease of Km-value Cellvibrio japonicus
H211N/D283H decrease of Km-value by about 50% Cellvibrio japonicus
W156A increase of Km-valueby almost 80% Cellvibrio japonicus
W162A increase of Km-value by about 50% Cellvibrio japonicus
W217A increase of Km-value by more than 100% Cellvibrio japonicus
Y285A dramatic decrease of Km-value Cellvibrio japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00066
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant E212A Cellvibrio japonicus
0.00074
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant Y285A Cellvibrio japonicus
0.0014
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant D283H Cellvibrio japonicus
0.0037
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant H211A/D283A Cellvibrio japonicus
0.0068
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant H211A Cellvibrio japonicus
0.0093
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, wild type Cellvibrio japonicus
0.01
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant H211N Cellvibrio japonicus
0.0141
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant W162A Cellvibrio japonicus
0.0149
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant W156A Cellvibrio japonicus
0.0166
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant D283A Cellvibrio japonicus
0.0205
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant W217A Cellvibrio japonicus
0.04
-
2,4-dinitrophenyl-beta-mannobioside pH 6.5, mutant H143A Cellvibrio japonicus

Organism

Organism UniProt Comment Textmining
Cellvibrio japonicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl-beta-mannobioside + H2O
-
Cellvibrio japonicus 2,4-dinitrophenol + beta-mannobiose
-
?

Subunits

Subunits Comment Organism
More Trp360 is critical in substrate binding at -1 subsite, Tyr285 works as nucleophile catalyst, Trp217 and Trp162 are important for the activity against mannooligosaccharides but less important for activity against polysaccharides Cellvibrio japonicus