Literature summary for 3.2.1.76 extracted from

Maita, N.; Taniguchi, H.; Sakuraba, H.
Crystallization, X-ray diffraction analysis and SIRAS phasing of human alpha-L-iduronidase (2012), Acta Crystallogr. Sect. F, 68, 1363-1366.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, 4 mg/ml protein, mixing of 0.008 ml of both protein solution and reservoir solution, the latter containing 18% w/v PEG 3350, 0.18 M K/Na-tartrate, 3% w/v PEG MME 5000, 0.02 M ammonium sulfate, and 0.01 M MES-Na, pH 6.5, 25°C, 2-3 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, mercury-derivative crystals by single-wavelength anomalous dispersion data, Hg peak wavelength	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, 4 mg/ml protein, mixing of 0.008 ml of both protein solution and reservoir solution, the latter containing 18% w/v PEG 3350, 0.18 M K/Na-tartrate, 3% w/v PEG MME 5000, 0.02 M ammonium sulfate, and 0.01 M MES-Na, pH 6.5, 25°C, 2-3 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, mercury-derivative crystals by single-wavelength anomalous dispersion data, Hg peak wavelength

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
69908	-	x * 69908, sequence calculation	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
dermatan sulfate + H2O	Homo sapiens	-	? + alpha-iduronic acid	-	?
glycosaminoglycan + H2O	Homo sapiens	-	? + alpha-iduronic acid	-	?
heparan sulfate + H2O	Homo sapiens	-	? + alpha-L-iduronic acid	-	?
additional information	Homo sapiens	the enzyme cleaves terminal alpha-iduronic acid residues from glycosaminoglycan, heparan sulfate and dermatan sulfate	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35475	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	six potential N-glycosylation sites	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
the commercial preparation of recombinant enzyme expressed in CHO cells is further purified by gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	recombinant enzyme expressed in CHO cells	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
dermatan sulfate + H2O	-	Homo sapiens	? + alpha-iduronic acid	-	?
glycosaminoglycan + H2O	-	Homo sapiens	? + alpha-iduronic acid	-	?
heparan sulfate + H2O	-	Homo sapiens	? + alpha-L-iduronic acid	-	?
additional information	the enzyme cleaves terminal alpha-iduronic acid residues from glycosaminoglycan, heparan sulfate and dermatan sulfate	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 69908, sequence calculation	Homo sapiens

Synonyms

Synonyms	Comment	Organism
alpha-L-iduronidase	-	Homo sapiens
hIDUA	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycosyl hydrolase family 39, GH39	Homo sapiens
malfunction	enzyme deficiency causes mucopolysaccharidosis type I, patients with MPS I developmental retardation, gross facial features, an enlarged and deformed skull, a small stature, corneal opacities, hepatosplenomegaly, valvular heart defects, thick skin, joint contractures and hernias	Homo sapiens