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Literature summary for 3.2.1.75 extracted from
- Purification and characterization of beta-1,6-glucanase of Streptomyces rochei application in the study of yeast cell wall proteins (2002), Biosci. Biotechnol. Biochem., 66, 2515-2519.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | use of enzyme in analysis of yeast cell wall protein | Streptomyces rochei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Streptomyces rochei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces rochei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces rochei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 83.3 | - |
pH 5.0, 30°C | Streptomyces rochei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, repeated freezing and thwaing severely damages enzyme activity | Streptomyces rochei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| curdlan + H2O | 0.3% of the activity against pustulan | Streptomyces rochei | ? | - |
? |  |
| laminarin + H2O | 0.5% of the activity against pustulan | Streptomyces rochei | ? | - |
? | |
| additional information | little activity against beta-(1-3)- or beta-(1-4)-linkages | Streptomyces rochei | ? | - |
? | |
| pustulan + H2O | - |
Streptomyces rochei | D-glucose + gentobiose + ? | main products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Streptomyces rochei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Streptomyces rochei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Streptomyces rochei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Streptomyces rochei |
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