Any feedback?
Literature summary for 3.2.1.75 extracted from
- Purification and characterization of an extracellular (1,6)-beta-glucanase from the filamentous fungus Acremonium persicinum (1996), Biochem. J., 316, 841-846.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxy-5-nitrobenzyl bromide | - |
Acremonium persicinum |  |
| Cu2+ | - |
Acremonium persicinum | |
| Dicyclohexylcarbodiimide | - |
Acremonium persicinum | |
| Hg2+ | - |
Acremonium persicinum | |
| KMnO4 | - |
Acremonium persicinum | |
| MgSO4 | - |
Acremonium persicinum | |
| Mn2+ | - |
Acremonium persicinum | |
| N-Acetylimidazole | - |
Acremonium persicinum | |
| N-bromosuccinimide | - |
Acremonium persicinum | |
| N-ethyl-5-phenylisoxazolium 3'-sulfonate | - |
Acremonium persicinum | |
| p-hydroxymercuribenzoate | - |
Acremonium persicinum | |
| Sn2+ | - |
Acremonium persicinum | |
| Zn2+ | - |
Acremonium persicinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | lutean 1.38 mg/ml, laminarin 1.67 mg/ml | Acremonium persicinum | |
| 0.0753 | - |
pustulan | assumed molecular weight for pustulan 17000 Da, or KM of 1.28 mg/ml | Acremonium persicinum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Acremonium persicinum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42700 | - |
x * 42700, SDS-PAGE | Acremonium persicinum |
| 44300 | - |
gel filtration | Acremonium persicinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acremonium persicinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 89fold | Acremonium persicinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Acremonium persicinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 146 | - |
- |
Acremonium persicinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 4 months, no loss of activity, -70°C, 12 months, no loss of activity | Acremonium persicinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | yeast glucans | Acremonium persicinum | glucose + laminari-oligosaccharides + gentio-oligosaccharides | - |
? | |
| laminarin + H2O | - |
Acremonium persicinum | gentiobiose + D-glucose | - |
? | |
| lutean + H2O | - |
Acremonium persicinum | D-glucose + gentiobiose + gentiotriose | - |
? | |
| pustulan + H2O | - |
Acremonium persicinum | glucose + gentiobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42700, SDS-PAGE | Acremonium persicinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
short-term stability up to 50°C | Acremonium persicinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Acremonium persicinum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | - |
Acremonium persicinum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
