Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenibacillus curdlanolyticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncation mutant enzymes X-CBM22, X-CBM3 and X-GH10 show 100%, 25.9% and 26.9% activity with barley beta-glucan, respectively, as compared to the wild type enzyme | Paenibacillus curdlanolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus curdlanolyticus | - |
- |
- |
Paenibacillus curdlanolyticus B-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Profinia affinity chromatography protein purification system | Paenibacillus curdlanolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Paenibacillus curdlanolyticus | D-glucose + ? | - |
? | |
barley beta-glucan + H2O | - |
Paenibacillus curdlanolyticus B-6 | D-glucose + ? | - |
? | |
additional information | the recombinant enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose | Paenibacillus curdlanolyticus | ? | - |
? | |
additional information | the recombinant enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose | Paenibacillus curdlanolyticus B-6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Paenibacillus curdlanolyticus |
? | x * 92804, calculated from amino acid sequence | Paenibacillus curdlanolyticus |
Synonyms | Comment | Organism |
---|---|---|
Xyn10E | bifunctional recombinant enzyme with xylanase and beta-glucanase activities | Paenibacillus curdlanolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
Barley beta-glucan | at pH 6.0, temperature not specified in the publication | Paenibacillus curdlanolyticus |