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Literature summary for 3.2.1.74 extracted from

  • Zeng, R.; Hu, Q.; Yin, X.Y.; Huang, H.; Yan, J.B.; Gong, Z.W.; Yang, Z.H.
    Cloning a novel endo-1,4-beta-D-glucanase gene from Trichoderma virens and heterologous expression in E. coli (2018), AMB Express, 6, 108 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trichoderma virens

Inhibitors

Inhibitors Comment Organism Structure
Ni2+ about 88% residual activity at 2.5 mM Trichoderma virens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ about 150% at 2.5 mM Trichoderma virens
Mn2+ about 160% at 2.5 mM Trichoderma virens
additional information not influenced by Zn2+, Ca2+, Mg2+ and Cu2+ Trichoderma virens

Organism

Organism UniProt Comment Textmining
Trichoderma virens A0A288W730
-
-
Trichoderma virens ZY-01 A0A288W730
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Trichoderma virens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Trichoderma virens ?
-
?
carboxymethyl cellulose + H2O
-
Trichoderma virens ZY-01 ?
-
?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Trichoderma virens

Synonyms

Synonyms Comment Organism
endo-1,4-beta-D-glucanase
-
Trichoderma virens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Trichoderma virens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 more than 75% activity between 20 and 75°C Trichoderma virens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Trichoderma virens

pH Range

pH Minimum pH Maximum Comment Organism
6 12 more than 60% activity between pH 6.0 and 12.0 Trichoderma virens