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Literature summary for 3.2.1.74 extracted from

  • Takashima, S.; Nakamura, A.; Masaki, H.; Uozumi, T.
    Purification and characterization of cellulases from Humicola grisea (1996), Biosci. Biotechnol. Biochem., 60, 77-82 .
No PubMed abstract available
Search for more literature for 3.2.1.74

Organism

Organism UniProt Comment Textmining
[Humicola] grisea var. thermoidea
-
-
-
[Humicola] grisea var. thermoidea IFO 9854
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Humicola] grisea var. thermoidea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 5.0, 50°C [Humicola] grisea var. thermoidea
2.57
-
 substrate carboxymethyl cellulose, pH 5.0, 50°C [Humicola] grisea var. thermoidea
8.09
-
 substrate 4-nitrophenyl beta-D-cellobioside, pH 5.0, 50°C [Humicola] grisea var. thermoidea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 [Humicola] grisea var. thermoidea 4-nitrophenol + beta-D-cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 4-nitrophenol + beta-D-cellobiose
-
 ?
carboxymethyl cellulose + H2O
-
 [Humicola] grisea var. thermoidea ?
-
 ?
carboxymethyl cellulose + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38500, SDS-PAGE [Humicola] grisea var. thermoidea