Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ca2+ | uncultured bacterium | |
Cu2+ | - |
uncultured bacterium | |
Mg2+ | the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+ | uncultured bacterium | |
Ni2+ | the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+ | uncultured bacterium | |
Zn2+ | the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Zn2+ | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.74 | - |
4-nitrophenyl beta-D-cellobioside | at 65°C in pH 5.5 citrate buffer | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the addition of 5 mM Zn2+, Co2+ and Cu2+ has no statistical difference on enzyme activity | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38400 | - |
x * 38400, calculated from amino acid sequence | uncultured bacterium |
40000 | - |
x * 40000, SDS-PAGE | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
from yak rumen | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resincolumn chromatography | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
using filter paper as substrate, at 60°C in pH 6.5 citrate buffer | uncultured bacterium |
0.2 | - |
using avicel as substrate, at 60°C in pH 6.5 citrate buffer | uncultured bacterium |
23.5 | - |
using phosphate acid swollen cellulose as substrate, at 60°C in pH 6.5 citrate buffer | uncultured bacterium |
52.9 | - |
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65°C in pH 5.5 citrate buffer | uncultured bacterium |
220 | - |
using carboxymethylcellulose as substrate, at 65°C in pH 5.5 citrate buffer | uncultured bacterium |
693 | - |
using lichenan as substrate, at 60°C in pH 6.5 citrate buffer | uncultured bacterium |
717 | - |
using barley glucan as substrate, at 60°C in pH 6.5 citrate buffer | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose | uncultured bacterium | 4-nitrophenol + beta-D-cellobiose | - |
? | |
avicel + H2O | 0.09% activity compared to carboxymethylcellulose | uncultured bacterium | cellobiose + ? | - |
? | |
barley glucan + H2O | 326% activity compared to carboxymethylcellulose | uncultured bacterium | ? | - |
? | |
carboxymethylcellulose + H2O | endo-beta-1,4-glucanase activity, 100% activity | uncultured bacterium | ? | - |
? | |
cellotetraose + H2O | - |
uncultured bacterium | cellobiose + cellotriose + D-glucose | cellobiose is the dominant product, while cellotriose and glucose are minor products | ? | |
filter paper + H2O | 0.02% activity compared to carboxymethylcellulose | uncultured bacterium | cellobiose + ? | - |
? | |
lichenan + H2O | 315% activity compared to carboxymethylcellulose | uncultured bacterium | ? | - |
? | |
additional information | no activity with laminarin, glucan, birchwood xylan, and 4-nitrophenyl beta-D-glucopyranside | uncultured bacterium | ? | - |
? | |
additional information | the cellulase Rucel5B possesses endo-beta-1,4-glucanase activity and exo-beta-1,4-glucanase activity | uncultured bacterium | ? | - |
? | |
phosphate acid swollen cellulose + H2O | 10.7% activity compared to carboxymethylcellulose | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | uncultured bacterium |
? | x * 38400, calculated from amino acid sequence | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
endo/exocellulase | - |
uncultured bacterium |
exo-bets-1,4-glucanase | - |
uncultured bacterium |
Rucel5B | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
using carboxymethylcellulose as substrate | uncultured bacterium |
65 | - |
using 4-nitrophenyl beta-D-cellobioside as substrate | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the melting temperature of the enzyme is at 60°C | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69.6 | - |
4-nitrophenyl beta-D-cellobioside | at 65°C in pH 5.5 citrate buffer | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | using 4-nitrophenyl beta-D-cellobioside as substrate | uncultured bacterium |
6.5 | - |
using carboxymethylcellulose as substrate | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40.1 | - |
4-nitrophenyl beta-D-cellobioside | at 65°C in pH 5.5 citrate buffer | uncultured bacterium |