Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.74 extracted from

  • Bao, L.; Huang, Q.; Chang, L.; Zhou, J.; Lu, H.
    Screening and characterization of a cellulase with endocellulase and exocellulase activity from yak rumen metagenome (2011), J. Mol. Catal. B, 73, 104-110.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ca2+ uncultured bacterium
Cu2+
-
uncultured bacterium
Mg2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+ uncultured bacterium
Ni2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+ uncultured bacterium
Zn2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Zn2+ uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.74
-
4-nitrophenyl beta-D-cellobioside at 65°C in pH 5.5 citrate buffer uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the addition of 5 mM Zn2+, Co2+ and Cu2+ has no statistical difference on enzyme activity uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38400
-
x * 38400, calculated from amino acid sequence uncultured bacterium
40000
-
x * 40000, SDS-PAGE uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
from yak rumen
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resincolumn chromatography uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
using filter paper as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
0.2
-
using avicel as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
23.5
-
using phosphate acid swollen cellulose as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
52.9
-
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65°C in pH 5.5 citrate buffer uncultured bacterium
220
-
using carboxymethylcellulose as substrate, at 65°C in pH 5.5 citrate buffer uncultured bacterium
693
-
using lichenan as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
717
-
using barley glucan as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose uncultured bacterium 4-nitrophenol + beta-D-cellobiose
-
?
avicel + H2O 0.09% activity compared to carboxymethylcellulose uncultured bacterium cellobiose + ?
-
?
barley glucan + H2O 326% activity compared to carboxymethylcellulose uncultured bacterium ?
-
?
carboxymethylcellulose + H2O endo-beta-1,4-glucanase activity, 100% activity uncultured bacterium ?
-
?
cellotetraose + H2O
-
uncultured bacterium cellobiose + cellotriose + D-glucose cellobiose is the dominant product, while cellotriose and glucose are minor products ?
filter paper + H2O 0.02% activity compared to carboxymethylcellulose uncultured bacterium cellobiose + ?
-
?
lichenan + H2O 315% activity compared to carboxymethylcellulose uncultured bacterium ?
-
?
additional information no activity with laminarin, glucan, birchwood xylan, and 4-nitrophenyl beta-D-glucopyranside uncultured bacterium ?
-
?
additional information the cellulase Rucel5B possesses endo-beta-1,4-glucanase activity and exo-beta-1,4-glucanase activity uncultured bacterium ?
-
?
phosphate acid swollen cellulose + H2O 10.7% activity compared to carboxymethylcellulose uncultured bacterium ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE uncultured bacterium
? x * 38400, calculated from amino acid sequence uncultured bacterium

Synonyms

Synonyms Comment Organism
endo/exocellulase
-
uncultured bacterium
exo-bets-1,4-glucanase
-
uncultured bacterium
Rucel5B
-
uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
using carboxymethylcellulose as substrate uncultured bacterium
65
-
using 4-nitrophenyl beta-D-cellobioside as substrate uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the melting temperature of the enzyme is at 60°C uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69.6
-
4-nitrophenyl beta-D-cellobioside at 65°C in pH 5.5 citrate buffer uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 using 4-nitrophenyl beta-D-cellobioside as substrate uncultured bacterium
6.5
-
using carboxymethylcellulose as substrate uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40.1
-
4-nitrophenyl beta-D-cellobioside at 65°C in pH 5.5 citrate buffer uncultured bacterium