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Literature summary for 3.2.1.74 extracted from
- Molecular cloning and characterization of a unique beta-glucosidase from Vibrio cholerae (2002), J. Biol. Chem., 277, 29555-29560.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Vibrio cholerae serotype O1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10% of enzyme inhibition at 20 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 |  |
| EGTA | 10% of enzyme inhibition at 20 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 | |
| KCl | 25% of enzyme inhibition at 0.5 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 | |
| NaCl | 25% of enzyme inhibition at 0.5 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
p-nitrophenyl glucoside | pH 6.5, at 37ºC | Vibrio cholerae serotype O1 | |
| 6.96 | - |
cellobiose | pH 6.5, at 37ºC | Vibrio cholerae serotype O1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Vibrio cholerae serotype O1 | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | NaCl and KCl gives optimum results at 10 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
calculation from sequence of amino acids | Vibrio cholerae serotype O1 |
| 65000 | - |
SDS-PAGE | Vibrio cholerae serotype O1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
- |
- |
| Vibrio cholerae serotype O1 E1 Tor N16961 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Vibrio cholerae serotype O1 |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography | Vibrio cholerae serotype O1 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Vibrio cholerae serotype O1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.323 | - |
pH 6.5, at 37ºC | Vibrio cholerae serotype O1 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20ºC or -80ºC, 0.5 mM dithiothreitol | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose | Vibrio cholerae serotype O1 | 2 D-glucose | - |
? | |
| cellobiose + H2O | no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose | Vibrio cholerae serotype O1 E1 Tor N16961 | 2 D-glucose | - |
? | |
| cellopentaose + H2O | - |
Vibrio cholerae serotype O1 | glucose + ? | - |
? | |
| cellopentaose + H2O | - |
Vibrio cholerae serotype O1 E1 Tor N16961 | glucose + ? | - |
? | |
| cellotetraose + 3 H2O | - |
Vibrio cholerae serotype O1 | 4 D-glucose | - |
? | |
| cellotetraose + 3 H2O | - |
Vibrio cholerae serotype O1 E1 Tor N16961 | 4 D-glucose | - |
? | |
| cellotriose + H2O | - |
Vibrio cholerae serotype O1 | glucose + cellobiose | - |
? | |
| cellotriose + H2O | - |
Vibrio cholerae serotype O1 E1 Tor N16961 | glucose + cellobiose | - |
? | |
| p-nitrophenyl cellobioside + H2O | p-nitrophenyl releasing after a lag period | Vibrio cholerae serotype O1 | p-nitrophenyl + glucose | - |
? | |
| p-nitrophenyl cellobioside + H2O | p-nitrophenyl releasing after a lag period | Vibrio cholerae serotype O1 E1 Tor N16961 | p-nitrophenyl + glucose | - |
? | |
| p-nitrophenyl glucoside + H2O | p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine) | Vibrio cholerae serotype O1 | p-nitrophenyl + glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,4-glucosidase | - |
Vibrio cholerae serotype O1 |
| beta-glucosidase | - |
Vibrio cholerae serotype O1 |
| BglA | - |
Vibrio cholerae serotype O1 |
| cellobiase | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 42 | - |
Vibrio cholerae serotype O1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 37 | stable for at least 1 h | Vibrio cholerae serotype O1 |
| 40 | - |
incubation for 3 h, 40% loss of enzyme activity | Vibrio cholerae serotype O1 |
| 70 | - |
incubation for 3 h, 100% loss of enzyme activity | Vibrio cholerae serotype O1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Vibrio cholerae serotype O1 |
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