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Literature summary for 3.2.1.74 extracted from

  • Irwin, D.C.; Zhang, S.; Wilson, D.B.
    Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca (2000), Eur. J. Biochem., 267, 4988-4997.
    View publication on PubMed
Search for more literature for 3.2.1.74

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli and Streptomyces lividans, expression in Streptomyces lividans Thermobifida fusca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermobifida fusca
-
-

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q9XCD4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Thermobifida fusca

Purification (Commentary)

Purification (Comment) Organism
cloned Cel48A, centrifugation, phenylmethanesulfonyl fluoride and ammonium sulfate fractionation, phenyl-Sepharose column, Q-Sepharose column Thermobifida fusca
native Cel48A, HPLC Thermobifida fusca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cloned Cel48A, 0.068 µmol/min/µmol, filter paper, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
additional information
-
 cloned Cel48A, 0.191 µmol/min/µmol, bacterial microcrystalline cellulose, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
additional information
-
 cloned Cel48A, 0.288 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, thin-layer analysis Thermobifida fusca
additional information
-
 cloned Cel48A, 0.292 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
additional information
-
 cloned Cel48A, 0.405 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
additional information
-
 cloned Cel48A, approximately 16 µmol/min/µmol, cellotetraose, pH 5.5, at 50ºC, thin-layer analysis Thermobifida fusca
additional information
-
 cloned Cel48A, approximately 9 µmol/min/µmol, cellopentaose, pH 5.5, at 50ºC, thin-layer analysis Thermobifida fusca
additional information
-
 native Cel48A, 17.9 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC Thermobifida fusca
additional information
-
 native Cel48A, 3.7 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenol cellobiose + H2O
-
 Thermobifida fusca ?
-
 ?
4-methylumbelliferyl beta-D-cellotrioside + H2O
-
 Thermobifida fusca 4-methylumbelliferone + cellotriose
-
 ?
4-methylumbelliferyl-beta-D-cellobioside + H2O
-
 Thermobifida fusca ?
-
 ?
bacterial microcrystalline cellulose + H2O
-
 Thermobifida fusca ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermobifida fusca ?
-
 ?
cellopentaose + H2O
-
 Thermobifida fusca ?
-
 ?
cellotetraose + H2O no activity with cellotriose and chitin Thermobifida fusca ?
-
 ?
filter paper
-
 Thermobifida fusca ?
-
 ?
swollen cellulose + H2O
-
 Thermobifida fusca cellobiose + cellotriose
-
 ?

Synonyms

Synonyms Comment Organism
Cel48A
-
 Thermobifida fusca
exocellulase
-
 Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 decreases markedly above 50ºC Thermobifida fusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00039
-
 2,4-dinitrophenol cellobiose pH 5.5 Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 50ºC Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
5 9 retaines more than 50% of activity, 50ºC Thermobifida fusca

pI Value

Organism Comment pI Value Maximum pI Value
Thermobifida fusca IEF gel
-
 4