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Literature summary for 3.2.1.74 extracted from

  • Ware, C.E.; Lachke, A.H.; Gregg, K.
    Mode of action and substrate specificity of a purified exo-1,4-beta-D-glucosidase cloned from the cellulolytic bacterium Ruminococcus albus AR67 (1990), Biochem. Biophys. Res. Commun., 171, 777-786.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
p-nitrophenyl-beta-D-cellobioside
-
Ruminococcus albus
4
-
p-nitrophenyl-beta-D-glucopyranoside
-
Ruminococcus albus
6
-
cellopentaose
-
Ruminococcus albus
6.7
-
cellotetraose
-
Ruminococcus albus
8.7
-
cellotriose
-
Ruminococcus albus
12.5
-
cellobiose
-
Ruminococcus albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Ruminococcus albus
64000
-
gel filtration Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus
-
AR67
-
Ruminococcus albus AR67
-
AR67
-

Purification (Commentary)

Purification (Comment) Organism
66.7fold Ruminococcus albus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
-
Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
Ruminococcus albus D-glucose + cellotetraose + cellotriose
-
?
cellobiose + H2O
-
Ruminococcus albus AR67 D-glucose + cellotetraose + cellotriose
-
?
cellodextrins + H2O
-
Ruminococcus albus D-glucose + cellotriose + cellobiose
-
?
cellopentaose + H2O
-
Ruminococcus albus D-glucose + ?
-
?
cellotetraose + H2O
-
Ruminococcus albus D-glucose + cellotriose + cellobiose
-
?
cellotriose + H2O
-
Ruminococcus albus D-glucose + cellobiose
-
?
esculin + H2O
-
Ruminococcus albus ?
-
?
gentiobiose + H2O
-
Ruminococcus albus ?
-
?
laminaribiose + H2O
-
Ruminococcus albus ?
-
?
laminaribiose + H2O
-
Ruminococcus albus AR67 ?
-
?
laminarin + H2O
-
Ruminococcus albus ?
-
?
lichenan + H2O
-
Ruminococcus albus ?
-
?
lichenan + H2O
-
Ruminococcus albus AR67 ?
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
-
Ruminococcus albus D-glucose + p-nitrophenyl-beta-D-glucoside
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
Ruminococcus albus 4-nitrophenol + D-glucose
-
?
salicin + H2O
-
Ruminococcus albus ?
-
?
salicin + H2O
-
Ruminococcus albus AR67 ?
-
?
trehalose + H2O slight hydrolysis Ruminococcus albus ?
-
?
trehalose + H2O slight hydrolysis Ruminococcus albus AR67 ?
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Ruminococcus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
10% activity loss after 3 h at 37°C Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Ruminococcus albus