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Literature summary for 3.2.1.74 extracted from

  • Hamada, N.; Ishikawa, K.; Fuse, N.; Kodaira, R.; Shimosaka, M.; Amano, Y.; Kanda, T.; Okazaki, M.
    Purification, characterization and gene analysis of exo-cellulase II (Ex-2) from the white rot basidiomycete Irpex lacteus (1999), J. Biosci. Bioeng., 87, 442-451.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
isoenzyme II Irpex lacteus
56000
-
isoenzyme I Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Driselase, from Irpex lacteus Irpex lacteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.5
-
bacterial cellulose powder, not dried, isoenzyme II Irpex lacteus
15.1
-
bacterial cellulose powder, not dried, isoenzyme I Irpex lacteus
20.9
-
avicel, isoenzyme II Irpex lacteus
21.1
-
isoenzyme I Irpex lacteus
21.1
-
avicel, isoenzyme I Irpex lacteus
25.5
-
carboxymethyl cellulose, isoenzyme I Irpex lacteus
26
-
carboxymethyl cellulose, isoenzyme II Irpex lacteus
27.1
-
p-nitrophenyl beta-D-lactoside, isoenzyme II Irpex lacteus
27.3
-
p-nitrophenyl beta-D-lactoside, isoenzyme I Irpex lacteus
33.2
-
p-nitrophenyl beta-D-celloside, isoenzyme II Irpex lacteus
34
-
p-nitrophenyl beta-D-celloside, isoenzyme I Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Avicel + H2O
-
Irpex lacteus D-glucose + cellotetraose + cellotriose + cellobiose
-
?
carboxymethyl cellulose + H2O
-
Irpex lacteus ?
-
?
cellobiose + H2O only isoenzyme I Irpex lacteus D-glucose + cellotetraose + cellotriose
-
?
cellotetraose + H2O
-
Irpex lacteus D-glucose + cellotriose + cellobiose
-
?
cellulose + H2O bacterial cellulose powder, not dried Irpex lacteus D-glucose + ?
-
?
p-nitrophenyl beta-D-lactoside + H2O
-
Irpex lacteus ?
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
-
Irpex lacteus D-glucose + p-nitrophenyl-beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
both isoenzymes Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
both isoenzymes stable below 50°C Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
both isoenzymes Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 isoenzyme I Irpex lacteus
3 5 isoenzyme II Irpex lacteus