KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.62 mg/ml with barley beta-glucan, pH 5.0, 60°C | Aspergillus niger | |
additional information | - |
additional information | 0.67 mg/ml with barley beta-glucan, pH 4.0, 75°C | Laetiporus sulphureus var. miniatus | |
additional information | - |
additional information | 0.69 mg/ml with barley beta-glucan, 1.11 mg/ml with oat beta-glucan, and 0.63 mg/ml with lichenan, pH 5.0, 60°C | Malbranchea cinnamomea | |
additional information | - |
additional information | 22.39 mg/ml with barley beta-glucan, pH 4.0-5.0, 50-60°C | Rhizopus microsporus var. microsporus | |
additional information | - |
additional information | 5.957 mg/ml with lichenan, pH 3.0, 50-60°C | Penicillium sp. 'occitanis' | |
additional information | - |
additional information | Km is 13.38 mg/ml and Vmax is 0.1429 mM/min/mg with lichenan, pH 4.8, 80°C | Rasamsonia emersonii | |
additional information | - |
additional information | Km is 2.4 mg/ml and Vmax is 9.981 mmol/min/mg, with barley beta-glucan, pH 7.0, 70°C | Paecilomyces sp. 'thermophila' | |
1.4 | - |
lichenan | pH 5.5, 60°C | Rhizomucor miehei | |
2 | - |
Barley beta-glucan | pH 5.5, 60°C | Rhizomucor miehei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus niger | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Aspergillus terreus | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Trichoderma reesei | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Rhizomucor miehei | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Rasamsonia emersonii | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Malbranchea cinnamomea | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Laetiporus sulphureus var. miniatus | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Orpinomyces sp. PC-2 | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Penicillium sp. 'occitanis' | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Paecilomyces sp. 'thermophila' | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Rhizopus microsporus var. microsporus | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Aspergillus niger US368 | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Rasamsonia emersonii CBS 841.70 | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Rhizomucor miehei CAU432 | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Trichoderma reesei GXC | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Penicillium sp. 'occitanis' Pol6 | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? | |
additional information | Aspergillus terreus ASKU 10 | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger US368 | - |
- |
- |
Aspergillus terreus | - |
- |
- |
Aspergillus terreus ASKU 10 | - |
- |
- |
Laetiporus sulphureus var. miniatus | - |
- |
- |
Malbranchea cinnamomea | - |
- |
- |
Orpinomyces sp. PC-2 | - |
- |
- |
Paecilomyces sp. 'thermophila' | E0XN39 | - |
- |
Penicillium sp. 'occitanis' | - |
- |
- |
Penicillium sp. 'occitanis' Pol6 | - |
- |
- |
Rasamsonia emersonii | - |
- |
- |
Rasamsonia emersonii CBS 841.70 | - |
- |
- |
Rhizomucor miehei | - |
- |
- |
Rhizomucor miehei | H9BZ70 | - |
- |
Rhizomucor miehei CAU432 | - |
- |
- |
Rhizopus microsporus var. microsporus | - |
- |
- |
Trichoderma reesei | - |
- |
- |
Trichoderma reesei GXC | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | solid state fermentation on oatmeal | Rhizomucor miehei | - |
additional information | solid state fermentation on wheat bran | Trichoderma reesei | - |
additional information | submerged fermentation with 0.5% chitin | Rhizopus microsporus var. microsporus | - |
additional information | submerged fermentation with 2% wheat bran/beet pulp (ratio 1:1) | Rasamsonia emersonii | - |
additional information | submerged fermentation with 2.5% corncob | Malbranchea cinnamomea | - |
additional information | submerged fermentation with 3% oat flour | Rhizomucor miehei | - |
additional information | submerged fermentation with 5% corncob | Paecilomyces sp. 'thermophila' | - |
mycelium | solid state fermentation on rice straw | Aspergillus terreus | - |
mycelium | submerged fermentation with 1.4g/l barley flour | Rasamsonia emersonii | - |
mycelium | submerged fermentation with 2% oat flour | Penicillium sp. 'occitanis' | - |
mycelium | submerged fermentation with 8% barley flour | Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1.5 U/ml, pH and temperature not specified in the publication, substrate barley beta-glucan | Rasamsonia emersonii |
additional information | - |
100.3 U/ml, substrate barley beta-glucan, pH 5.0, 60°C | Malbranchea cinnamomea |
additional information | - |
135.6 U/ml, substrate barley beta-glucan, pH 7.0, 70°C | Paecilomyces sp. 'thermophila' |
additional information | - |
2.2 U/ml, pH 5.0, 60°C, substrate barley beta-glucan | Aspergillus niger |
additional information | - |
20.3 U/ml, pH 4.8, 80°C, substrate barley beta-glucan | Rasamsonia emersonii |
additional information | - |
20025 U/gds, pH 5.5, 60°C | Rhizomucor miehei |
additional information | - |
45.0 U/ml, pH 3.0, 50-60°C, substrate lichenan | Penicillium sp. 'occitanis' |
additional information | - |
4504 U/gds | Aspergillus terreus |
additional information | - |
6230 U/ml, pH and temperature not specified in the publication | Rhizomucor miehei |
additional information | - |
8.5 U/ml, pH 4.0-5.0, 50-60°C, substrate barley beta-glucan | Rhizopus microsporus var. microsporus |
additional information | - |
9023.62 U/gds, pH and temperature not specified in the publication | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Aspergillus niger | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Rasamsonia emersonii | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Malbranchea cinnamomea | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Laetiporus sulphureus var. miniatus | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Paecilomyces sp. 'thermophila' | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Rhizomucor miehei | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Rhizopus microsporus var. microsporus | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Aspergillus niger US368 | ? | - |
? | |
barley beta-glucan + H2O | the main final hydrolysis products from barley beta-glucan are the trisaccharide and the tetrasaccharide, which is typical for true bacterial lichenases | Rasamsonia emersonii CBS 841.70 | ? | - |
? | |
lichenan + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
lichenan + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
lichenan + H2O | - |
Penicillium sp. 'occitanis' | ? | - |
? | |
lichenan + H2O | - |
Rhizomucor miehei | ? | - |
? | |
lichenan + H2O | - |
Rasamsonia emersonii CBS 841.70 | ? | - |
? | |
lichenan + H2O | - |
Penicillium sp. 'occitanis' Pol6 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Aspergillus niger | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Aspergillus terreus | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Trichoderma reesei | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Rhizomucor miehei | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Rasamsonia emersonii | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Malbranchea cinnamomea | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Laetiporus sulphureus var. miniatus | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Orpinomyces sp. PC-2 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Penicillium sp. 'occitanis' | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Paecilomyces sp. 'thermophila' | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Rhizopus microsporus var. microsporus | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Aspergillus niger US368 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Rasamsonia emersonii CBS 841.70 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Rhizomucor miehei CAU432 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Trichoderma reesei GXC | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Penicillium sp. 'occitanis' Pol6 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanases or lichenases hydrolyze beta-glucans containing beta-1,3 and beta-1,4 linkages, such as cereal beta-glucans and lichenan | Aspergillus terreus ASKU 10 | ? | - |
? | |
oat beta-glucan + H2O | - |
Malbranchea cinnamomea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000 | Aspergillus niger |
? | x * 52000 | Laetiporus sulphureus var. miniatus |
? | x * 20000 | Penicillium sp. 'occitanis' |
? | x * 26000 | Orpinomyces sp. PC-2 |
? | x * 33700 | Rhizomucor miehei |
? | x * 33700 | Rhizopus microsporus var. microsporus |
? | x * 34600 | Paecilomyces sp. 'thermophila' |
? | x * 40700 | Rasamsonia emersonii |
? | x * 44700 | Malbranchea cinnamomea |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Aspergillus niger |
beta-1,3-1,4-glucanase | - |
Aspergillus terreus |
beta-1,3-1,4-glucanase | - |
Trichoderma reesei |
beta-1,3-1,4-glucanase | - |
Rhizomucor miehei |
beta-1,3-1,4-glucanase | - |
Rasamsonia emersonii |
beta-1,3-1,4-glucanase | - |
Malbranchea cinnamomea |
beta-1,3-1,4-glucanase | - |
Laetiporus sulphureus var. miniatus |
beta-1,3-1,4-glucanase | - |
Orpinomyces sp. PC-2 |
beta-1,3-1,4-glucanase | - |
Penicillium sp. 'occitanis' |
beta-1,3-1,4-glucanase | - |
Paecilomyces sp. 'thermophila' |
beta-1,3-1,4-glucanase | - |
Rhizopus microsporus var. microsporus |
Lichenase | - |
Aspergillus niger |
Lichenase | - |
Aspergillus terreus |
Lichenase | - |
Trichoderma reesei |
Lichenase | - |
Rhizomucor miehei |
Lichenase | - |
Rasamsonia emersonii |
Lichenase | - |
Malbranchea cinnamomea |
Lichenase | - |
Laetiporus sulphureus var. miniatus |
Lichenase | - |
Orpinomyces sp. PC-2 |
Lichenase | - |
Penicillium sp. 'occitanis' |
Lichenase | - |
Paecilomyces sp. 'thermophila' |
Lichenase | - |
Rhizopus microsporus var. microsporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Orpinomyces sp. PC-2 |
50 | 60 | - |
Penicillium sp. 'occitanis' |
50 | 60 | - |
Rhizopus microsporus var. microsporus |
55 | - |
- |
Malbranchea cinnamomea |
60 | - |
- |
Aspergillus niger |
60 | - |
- |
Rhizomucor miehei |
70 | - |
- |
Paecilomyces sp. 'thermophila' |
75 | - |
- |
Laetiporus sulphureus var. miniatus |
80 | - |
- |
Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, stable | Paecilomyces sp. 'thermophila' |
50 | - |
2 h, stable | Rhizopus microsporus var. microsporus |
60 | 70 | half-lives are 80 min at 60°C and 21 min at 70°C | Penicillium sp. 'occitanis' |
70 | - |
half-life is 30 min | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Penicillium sp. 'occitanis' |
4 | - |
- |
Laetiporus sulphureus var. miniatus |
4 | 5 | - |
Rhizopus microsporus var. microsporus |
4.8 | - |
- |
Rasamsonia emersonii |
5 | - |
- |
Aspergillus niger |
5.5 | - |
- |
Rhizomucor miehei |
6 | - |
- |
Orpinomyces sp. PC-2 |
7 | - |
- |
Paecilomyces sp. 'thermophila' |
10 | - |
- |
Malbranchea cinnamomea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | stable at | Aspergillus niger |
4 | 10 | stable at | Malbranchea cinnamomea |
4 | 10 | stable at | Paecilomyces sp. 'thermophila' |
6 | 10 | stable at | Penicillium sp. 'occitanis' |