Any feedback?
Literature summary for 3.2.1.73 extracted from
- Characterization of a fungal thermostable endoglucanase from Chinese Nong-flavor daqu by metatranscriptomic method (2019), Int. J. Biol. Macromol., 121, 183-190 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene NFEg16A, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as intracellular soluble protein | uncultured Paecilomyces |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 35% inhibition at 10 mM | uncultured Paecilomyces |  |
| Ba2+ | 18% inhibition at 10 mM | uncultured Paecilomyces | |
| Co2+ | 43% inhibition at 10 mM | uncultured Paecilomyces | |
| Cu2+ | 35% inhibition at 10 mM | uncultured Paecilomyces | |
| Fe3+ | complete inhibition at 10 mM | uncultured Paecilomyces | |
| Hg2+ | complete inhibition at 10 mM | uncultured Paecilomyces | |
| K+ | 15% inhibition at 10 mM | uncultured Paecilomyces | |
| Li+ | 8.5% inhibition at 10 mM | uncultured Paecilomyces | |
| Mg2+ | 12% inhibition at 10 mM | uncultured Paecilomyces | |
| SDS | 14% inhibition at 10 mM | uncultured Paecilomyces | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme NFEg16A has a signal peptide | uncultured Paecilomyces | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no or poor effects by NaCl, NH4Cl, NiCl2, ZnSO4 and EDTA | uncultured Paecilomyces |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured Paecilomyces | A0A386YJ14 | uncultured bacterium from Chinese Nong-flavor daqu | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | uncultured Paecilomyces |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85.3 | - |
purified recombinant enzyme, pH 6.5, 60°C, substrate barley beta-glucan | uncultured Paecilomyces |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | enzyme NFEg16A efficiently hydrolyzes the substrate at a high concentration of 15 mg/mL, and releases glucose and cellobiose as its main end products | uncultured Paecilomyces | beta-D-glucose + cellobiose + ? | - |
? | |
| additional information | enzyme NFEg16A does not show clear activity against nature product of laminarin and carboxymethylcellulose-Na, but shows activity on barley beta-glucan, which might indicate that it is a true beta-1,3-1,4-glucanase (EC 3.2.1.73) | uncultured Paecilomyces | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33300, recombinant His-tagged enzyme, SDS-PAGE | uncultured Paecilomyces |
| More | the enzyme has an endo-1,3-1,4-beta-glucanase domain | uncultured Paecilomyces |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-glucanase | - |
uncultured Paecilomyces |
| endotype beta-1,3-1,4-glucanase | - |
uncultured Paecilomyces |
| NFEg16A | - |
uncultured Paecilomyces |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | uncultured Paecilomyces |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 85 | over 80% of maximal activity within this range | uncultured Paecilomyces |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant enzyme, completely stable for 8 h, and loss of 20% activity after 22 h | uncultured Paecilomyces |
| 80 | 90 | purified recombinant enzyme, 70% and 40% activity remaining after 2 h at 80°C and 90°C, respectively, half-lives of the enzyme are 3.4 h and 1.4 h at 80°C and 90°C, respectively | uncultured Paecilomyces |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme | uncultured Paecilomyces |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | over 60% of maximal activity within this range | uncultured Paecilomyces |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | purified recombinant enzyme, 30 min, over 80% activity remaining | uncultured Paecilomyces |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme has an endo-1,3-1,4-beta-glucanase domain | uncultured Paecilomyces |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
