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Literature summary for 3.2.1.73 extracted from

  • Niu, C.; Zhu, L.; Hill, A.; Alex Speers, R.; Li, Q.
    Construction of a highly thermostable 1,3-1,4-beta-glucanase by combinational mutagenesis and its potential application in the brewing industry (2017), Biotechnol. Lett., 39, 113-122 .
    View publication on PubMed

Application

Application Comment Organism
food industry the addition of mutant K20S/N31c/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P in congress mashing decreases the filtration time and viscosity by 21.3 and 9.6 %, respectively Bacillus tequilensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus tequilensis

Protein Variants

Protein Variants Comment Organism
K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P mutant with increased catalytic activity and thermostability Bacillus tequilensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
azo-barley glucan mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C Bacillus tequilensis
0.3
-
azo-barley glucan wild-type, pH 6.5, 40°C Bacillus tequilensis

Organism

Organism UniProt Comment Textmining
Bacillus tequilensis A0A1W6AKT2 sequence of mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2490
-
wild-type, pH 6.5, 40°C Bacillus tequilensis
4274
-
mutant K20S/N31C/CS40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C Bacillus tequilensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azo-barley glucan + H2O
-
Bacillus tequilensis ?
-
?

Synonyms

Synonyms Comment Organism
BglTO
-
Bacillus tequilensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
wild-type Bacillus tequilensis
70
-
mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P Bacillus tequilensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 wild-type, more than 80% of maximum activity Bacillus tequilensis
35 70 mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, more than 80% of maximum activity Bacillus tequilensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
137
-
azo-barley glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
189
-
azo-barley glucan mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C Bacillus tequilensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
462
-
azo-barley glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
691
-
azo-barley glucan mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C Bacillus tequilensis