Application | Comment | Organism |
---|---|---|
food industry | the addition of mutant K20S/N31c/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P in congress mashing decreases the filtration time and viscosity by 21.3 and 9.6 %, respectively | Bacillus tequilensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus tequilensis |
Protein Variants | Comment | Organism |
---|---|---|
K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | mutant with increased catalytic activity and thermostability | Bacillus tequilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
azo-barley glucan | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis | |
0.3 | - |
azo-barley glucan | wild-type, pH 6.5, 40°C | Bacillus tequilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus tequilensis | A0A1W6AKT2 | sequence of mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2490 | - |
wild-type, pH 6.5, 40°C | Bacillus tequilensis |
4274 | - |
mutant K20S/N31C/CS40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azo-barley glucan + H2O | - |
Bacillus tequilensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BglTO | - |
Bacillus tequilensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
wild-type | Bacillus tequilensis |
70 | - |
mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | Bacillus tequilensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | wild-type, more than 80% of maximum activity | Bacillus tequilensis |
35 | 70 | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, more than 80% of maximum activity | Bacillus tequilensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
137 | - |
azo-barley glucan | wild-type, pH 6.5, 40°C | Bacillus tequilensis | |
189 | - |
azo-barley glucan | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
462 | - |
azo-barley glucan | wild-type, pH 6.5, 40°C | Bacillus tequilensis | |
691 | - |
azo-barley glucan | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis |