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Literature summary for 3.2.1.73 extracted from
- Purification and characterization of a novel lichenase from Bacillus licheniformis GZ-2 (2016), Biotechnol. Appl. Biochem., 63, 249-256 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 115% of initial activity | Bacillus licheniformis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 77% residual activity | Bacillus licheniformis | |
| Fe3+ | 1 mM, 65% residual activity | Bacillus licheniformis | |
| hexadecyltrimethylammonium bromide | 1 mM, no residual activity | Bacillus licheniformis | |
| Mn2+ | 1 mM, 87% residual activity | Bacillus licheniformis | |
| N-bromosuccinimide | 1 mM, no residual activity | Bacillus licheniformis | |
| SDS | 1 mM, no residual activity | Bacillus licheniformis | |
| Zn2+ | 1 mM, 87% residual activity | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
lichenan | Km value 5.11 mg/ml, pH 6.5, 60°C | Bacillus licheniformis | |
| additional information | - |
Barley beta-glucan | Km value 7.42 mg/ml, pH 6.5, 60°C | Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 111% of initial activity | Bacillus licheniformis | |
| Co2+ | 1 mM, 116% of initial activity | Bacillus licheniformis | |
| Ni2+ | 1 mM, 114% of initial activity | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis GZ-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8231 | - |
pH 6.5, 60°C | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| barley beta-glucan + H2O | - |
Bacillus licheniformis GZ-2 | ? | - |
? | |
| lichenan + H2O | - |
Bacillus licheniformis | cellotriose + cellotetraose + ? | - |
? | |
| lichenan + H2O | - |
Bacillus licheniformis GZ-2 | cellotriose + cellotetraose + ? | - |
? | |
| additional information | enzyme exhibits strict specificity for beta-1,3-1,4-D-glucans | Bacillus licheniformis | ? | - |
? | |
| additional information | enzyme exhibits strict specificity for beta-1,3-1,4-D-glucans | Bacillus licheniformis GZ-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE, x * 24400, calculated | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bacillus licheniformis |
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