Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 7.5% (v/v) 2-methyl-2,4-pentanediol, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, and 0.1 M Tris (base)/Bicine, pH 8.5 | Paenibacillus sp. X4 |
to 1.8 A resolution | Paenibacillus sp. X4 |
Protein Variants | Comment | Organism |
---|---|---|
C17R/Q87T/D152G/Y307H/V330A/N344D | 2.1fold increase in specific activity | Paenibacillus sp. X4 |
C17R/Q87T/D152G/Y307H/V330A/N344D | the mutant shows 200.8% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
E149D/V370E | the mutant shows 59.9% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
K12R/F30L/N114D/N252D/T348S/N380S | the mutant shows 62.1% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
additional information | truncated mutants, which comprise 360, 286, and 215 amino acid residues instead of the 409 residues of the wild type, show complete loss of enzymatic activity | Paenibacillus sp. X4 |
N197D/M322V | 1.2fold increase in specific activity | Paenibacillus sp. X4 |
N197D/M322V | the mutant shows 144.7% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
N4S | the mutant shows 75.3% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
S11R/T210S | the mutant shows 231.3% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
Y389F/ K395R | the mutant shows 81% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Paenibacillus sp. X4 | |
Fe2+ | - |
Paenibacillus sp. X4 | |
Mn2+ | - |
Paenibacillus sp. X4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
barley beta-D-glucan | Km value of wild-type 13.5 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 13.3 mg/ml, respectively, pH 5.0, 60°C | Paenibacillus sp. X4 | |
additional information | - |
barley beta-D-glucan | Km value of wild-type 19.7 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 16.3 mg/ml, respectively, pH 5.0, 40°C | Paenibacillus sp. X4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Paenibacillus sp. X4 | |
additional information | not influenced by Na+, K+, Mg2+, or Zn2+ | Paenibacillus sp. X4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. X4 | A0A088BCU2 | - |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap Q column chromatography and CHT-II column chromatography | Paenibacillus sp. X4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
607 | - |
wild-type, pH 5.0, 40°C | Paenibacillus sp. X4 |
724 | - |
mutant N197D/M322V, pH 5.0, 40°C | Paenibacillus sp. X4 |
1282 | - |
mutant C17R/Q87T/D152G/Y307H/V330A/N344D, pH 5.0, 40°C | Paenibacillus sp. X4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-D-glucan + H2O | - |
Paenibacillus sp. X4 | ? | - |
? | |
barley beta-glucan + H2O | 100% activity | Paenibacillus sp. X4 | ? | - |
? | |
carboxymethyl cellulose + H2O | 22.4% activity compared to barley beta-glucan | Paenibacillus sp. X4 | ? | - |
? | |
laminarin + H2O | 0.12% activity compared to barley beta-glucan | Paenibacillus sp. X4 | ? | - |
? | |
lichenan + H2O | 136.4% activity compared to barley beta-glucan | Paenibacillus sp. X4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Paenibacillus sp. X4 |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Paenibacillus sp. X4 |
BGlc8H | - |
Paenibacillus sp. X4 |
Lic8H | - |
Paenibacillus sp. X4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Paenibacillus sp. X4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
after 30 min and 1 h at 60°C, the enzyme retains about 60% and 35% of activity, respectively | Paenibacillus sp. X4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
235 | - |
Barley beta-glucan | wild type enzyme, at pH 5.0 and 60°C | Paenibacillus sp. X4 | |
710 | - |
Barley beta-glucan | mutant enzyme C17R/Q87T/D152G/Y307H/V330A/N344D, at pH 5.0 and 60°C | Paenibacillus sp. X4 | |
2067 | - |
Barley beta-glucan | wild type enzyme, at pH 5.0 and 40°C | Paenibacillus sp. X4 | |
3628 | - |
Barley beta-glucan | mutant enzyme C17R/Q87T/D152G/Y307H/V330A/N344D, at pH 5.0 and 40°C | Paenibacillus sp. X4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Paenibacillus sp. X4 |