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Literature summary for 3.2.1.73 extracted from
- Improvement of enzyme activity of beta-1,3-1,4-glucanase from Paenibacillus sp. X4 by error-prone PCR and structural insights of mutated residues (2017), Appl. Microbiol. Biotechnol., 101, 4073-4083 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 7.5% (v/v) 2-methyl-2,4-pentanediol, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, and 0.1 M Tris (base)/Bicine, pH 8.5 | Paenibacillus sp. X4 |
| to 1.8 A resolution | Paenibacillus sp. X4 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C17R/Q87T/D152G/Y307H/V330A/N344D | 2.1fold increase in specific activity | Paenibacillus sp. X4 |
| C17R/Q87T/D152G/Y307H/V330A/N344D | the mutant shows 200.8% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
| E149D/V370E | the mutant shows 59.9% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
| K12R/F30L/N114D/N252D/T348S/N380S | the mutant shows 62.1% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
| additional information | truncated mutants, which comprise 360, 286, and 215 amino acid residues instead of the 409 residues of the wild type, show complete loss of enzymatic activity | Paenibacillus sp. X4 |
| N197D/M322V | 1.2fold increase in specific activity | Paenibacillus sp. X4 |
| N197D/M322V | the mutant shows 144.7% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
| N4S | the mutant shows 75.3% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
| S11R/T210S | the mutant shows 231.3% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
| Y389F/ K395R | the mutant shows 81% activity at pH 5.5 and 50°C compared to the wild type enzyme | Paenibacillus sp. X4 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Paenibacillus sp. X4 |  |
| Fe2+ | - |
Paenibacillus sp. X4 | |
| Mn2+ | - |
Paenibacillus sp. X4 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
barley beta-D-glucan | Km value of wild-type 13.5 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 13.3 mg/ml, respectively, pH 5.0, 60°C | Paenibacillus sp. X4 | |
| additional information | - |
barley beta-D-glucan | Km value of wild-type 19.7 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 16.3 mg/ml, respectively, pH 5.0, 40°C | Paenibacillus sp. X4 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Paenibacillus sp. X4 | |
| additional information | not influenced by Na+, K+, Mg2+, or Zn2+ | Paenibacillus sp. X4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus sp. X4 | A0A088BCU2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hi-Trap Q column chromatography and CHT-II column chromatography | Paenibacillus sp. X4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 607 | - |
wild-type, pH 5.0, 40°C | Paenibacillus sp. X4 |
| 724 | - |
mutant N197D/M322V, pH 5.0, 40°C | Paenibacillus sp. X4 |
| 1282 | - |
mutant C17R/Q87T/D152G/Y307H/V330A/N344D, pH 5.0, 40°C | Paenibacillus sp. X4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-D-glucan + H2O | - |
Paenibacillus sp. X4 | ? | - |
? | |
| barley beta-glucan + H2O | 100% activity | Paenibacillus sp. X4 | ? | - |
? | |
| carboxymethyl cellulose + H2O | 22.4% activity compared to barley beta-glucan | Paenibacillus sp. X4 | ? | - |
? | |
| laminarin + H2O | 0.12% activity compared to barley beta-glucan | Paenibacillus sp. X4 | ? | - |
? | |
| lichenan + H2O | 136.4% activity compared to barley beta-glucan | Paenibacillus sp. X4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Paenibacillus sp. X4 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Paenibacillus sp. X4 |
| BGlc8H | - |
Paenibacillus sp. X4 |
| Lic8H | - |
Paenibacillus sp. X4 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Paenibacillus sp. X4 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
after 30 min and 1 h at 60°C, the enzyme retains about 60% and 35% of activity, respectively | Paenibacillus sp. X4 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 235 | - |
Barley beta-glucan | wild type enzyme, at pH 5.0 and 60°C | Paenibacillus sp. X4 | |
| 710 | - |
Barley beta-glucan | mutant enzyme C17R/Q87T/D152G/Y307H/V330A/N344D, at pH 5.0 and 60°C | Paenibacillus sp. X4 | |
| 2067 | - |
Barley beta-glucan | wild type enzyme, at pH 5.0 and 40°C | Paenibacillus sp. X4 | |
| 3628 | - |
Barley beta-glucan | mutant enzyme C17R/Q87T/D152G/Y307H/V330A/N344D, at pH 5.0 and 40°C | Paenibacillus sp. X4 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Paenibacillus sp. X4 |
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