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Literature summary for 3.2.1.73 extracted from

  • Pei, H.; Guo, X.; Yang, W.; Lv, J.; Chen, Y.; Cao, Y.
    Directed evolution of a beta-1,3-1,4-glucanase from Bacillus subtilis MA139 for improving thermal stability and other characteristics (2015), J. Basic Microbiol., 55, 869-878.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
M44V/N53H mutant displays best ability to tolerate an acid environment and improved thermal stability Bacillus subtilis
M44V/N53H mutant displays improved thermal stability Bacillus subtilis
T113S mutant displays improved thermal stability Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, wild-type, complete loss of activity Bacillus subtilis
EDTA 10 mM, wild-type, 86% residual activity Bacillus subtilis
Mn2+ 10 mM, wild-type, 36% residual activity Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
lichenin Km value of wild-type 0.91 mg/ml, mutant T113S 0.69 mg/ml, mutant M44V/N53H 0.67 mg/ml, mutant N157D 0.70 mg/ml, respectively, pH 6.4, 40°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 10 mM, wild-type, 112% of initial activity Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis A8CGP1
-
-
Bacillus subtilis MA139 A8CGP1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenin + H2O
-
Bacillus subtilis ?
-
?
lichenin + H2O
-
Bacillus subtilis MA139 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
wild-type and mutants T113S, N157D Bacillus subtilis
50
-
mutant M44V/N53H Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
4 min, complete loss of activtiy for wild-type, 10 min, 60% residual activity for mutant T113S Bacillus subtilis
90
-
10 min, 40% residual activity for mutant T113S Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
wild-type Bacillus subtilis
6.6
-
mutants T113S, N157D Bacillus subtilis
7
-
mutant M44V/N53H Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
1 h, wild-type 5% residual activity, mutant T113S 40% residual activity, mutant M44V/N53H 50% residual activity, mutant M44V/N53H 80% residual activity Bacillus subtilis