Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
M44V/N53H | mutant displays best ability to tolerate an acid environment and improved thermal stability | Bacillus subtilis |
M44V/N53H | mutant displays improved thermal stability | Bacillus subtilis |
T113S | mutant displays improved thermal stability | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM, wild-type, complete loss of activity | Bacillus subtilis | |
EDTA | 10 mM, wild-type, 86% residual activity | Bacillus subtilis | |
Mn2+ | 10 mM, wild-type, 36% residual activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
lichenin | Km value of wild-type 0.91 mg/ml, mutant T113S 0.69 mg/ml, mutant M44V/N53H 0.67 mg/ml, mutant N157D 0.70 mg/ml, respectively, pH 6.4, 40°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | 10 mM, wild-type, 112% of initial activity | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | A8CGP1 | - |
- |
Bacillus subtilis MA139 | A8CGP1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lichenin + H2O | - |
Bacillus subtilis | ? | - |
? | |
lichenin + H2O | - |
Bacillus subtilis MA139 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
wild-type and mutants T113S, N157D | Bacillus subtilis |
50 | - |
mutant M44V/N53H | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
4 min, complete loss of activtiy for wild-type, 10 min, 60% residual activity for mutant T113S | Bacillus subtilis |
90 | - |
10 min, 40% residual activity for mutant T113S | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
wild-type | Bacillus subtilis |
6.6 | - |
mutants T113S, N157D | Bacillus subtilis |
7 | - |
mutant M44V/N53H | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
1 h, wild-type 5% residual activity, mutant T113S 40% residual activity, mutant M44V/N53H 50% residual activity, mutant M44V/N53H 80% residual activity | Bacillus subtilis |