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Literature summary for 3.2.1.73 extracted from

  • Chaari, F.; Belghith-Fendri, L.; Blibech, M.; Driss, D.; Ellouzi, S.; Sameh, M.; Ellouz-Chaabouni, S.
    Biochemical characterization of a lichenase from Penicillium occitanis Pol6 and its potential application in the brewing industry (2014), Process Biochem., 49, 1040-1046.
No PubMed abstract available

Application

Application Comment Organism
food industry the enzyme is a good candidate in the malting and brewing industry reducing the filtration time and viscosity of mash from barley grains, overview Penicillium sp. 'occitanis'

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 40% inhibition at 5 mM Penicillium sp. 'occitanis'
Cu2+ 40% inhibition at 5 mM Penicillium sp. 'occitanis'
EDTA 52% inhibition at 5 mM Penicillium sp. 'occitanis'
Hg2+ complete inhibition at 5 mM Penicillium sp. 'occitanis'
Mg2+ 90% inhibition at 5 mM Penicillium sp. 'occitanis'
Mn2+ complete inhibition at 5 mM Penicillium sp. 'occitanis'
Zn2+ complete inhibition at 5 mM Penicillium sp. 'occitanis'

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 5.96 mg/ml with lichenan at pH 5.5, 50°C Penicillium sp. 'occitanis'

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Penicillium sp. 'occitanis'
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 8% activation at 5 mM Penicillium sp. 'occitanis'
Fe2+ 50% activation at 5 mM Penicillium sp. 'occitanis'
K+ 11% activation at 5 mM Penicillium sp. 'occitanis'
Na+ 28% activation at 5 mM Penicillium sp. 'occitanis'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
x * 20000, SDS-PAGE Penicillium sp. 'occitanis'

Organism

Organism UniProt Comment Textmining
Penicillium sp. 'occitanis'
-
a hypercellulolytic mutant strain
-
Penicillium sp. 'occitanis' Pol6
-
a hypercellulolytic mutant strain
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 0.63fold from cell-free culture supernatant by heat treatment at 50°C for 10 min, anion exchange chromatography, lyophilization, and gel filtration to homogeneity Penicillium sp. 'occitanis'

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain Pol6 produces high level of extracellular lichenase when grown in a medium containing oat flour (2%, w/v) at 30°C for 7 days. Highest lichenases activity is obtained on the cellulose (50 U/ml) and oat flour (45 U/ml) as a carbon sources Penicillium sp. 'occitanis'
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.66
-
purified native enzyme, pH 5.5, 50°C, substrate lichenan Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O best substrate Penicillium sp. 'occitanis' cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides, mainly trisaccharide and tetrasaccharide ?
barley beta-glucan + H2O best substrate Penicillium sp. 'occitanis' Pol6 cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides, mainly trisaccharide and tetrasaccharide ?
lichenan + H2O 93.5% activity compared to barley beta-glucan Penicillium sp. 'occitanis' cellotriose + cellotetraose + ? oligosaccharides, mainly trisaccharide and tetrasaccharide ?
lichenan + H2O 93.5% activity compared to barley beta-glucan Penicillium sp. 'occitanis' Pol6 cellotriose + cellotetraose + ? oligosaccharides, mainly trisaccharide and tetrasaccharide ?
additional information no activity with carboxymethyl cellulose, laminarin, xylan, scleroglucan, and starch, substrate specificity, overview. The enzyme is inactive toward alpha-1,4, alpha-1,6, beta-1,4 and beta-1,6 linkages Penicillium sp. 'occitanis' ?
-
?
additional information no activity with carboxymethyl cellulose, laminarin, xylan, scleroglucan, and starch, substrate specificity, overview. The enzyme is inactive toward alpha-1,4, alpha-1,6, beta-1,4 and beta-1,6 linkages Penicillium sp. 'occitanis' Pol6 ?
-
?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Penicillium sp. 'occitanis'

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-D-glucanase
-
Penicillium sp. 'occitanis'
EGL
-
Penicillium sp. 'occitanis'
Lichenase
-
Penicillium sp. 'occitanis'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
Penicillium sp. 'occitanis'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified native enzyme, 75% activity remaining after 120 min Penicillium sp. 'occitanis'
50
-
purified native enzyme, 60% activity remaining after 120 min Penicillium sp. 'occitanis'
60
-
purified native enzyme, half-life is 80 min Penicillium sp. 'occitanis'
70
-
purified native enzyme, half-life is 21 min Penicillium sp. 'occitanis'

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.707
-
lichenan pH 5.5, 50°C Penicillium sp. 'occitanis'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Penicillium sp. 'occitanis'