Cloned (Comment) | Organism |
---|---|
gene bg1314, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21 | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is halotolerant | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26800 | - |
x * 26800, about, sequence calculation | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-1,4-glucan + H2O | Bacillus sp. (in: Bacteria) | beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose | 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose | - |
? | |
beta-1,3-1,4-glucan + H2O | Bacillus sp. (in: Bacteria) SJ-10 | beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose | 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose | - |
? | |
additional information | Bacillus sp. (in: Bacteria) | beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans | ? | - |
? | |
additional information | Bacillus sp. (in: Bacteria) SJ-10 | beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | I1W007 | gene bg1314 | - |
Bacillus sp. (in: Bacteria) SJ-10 | I1W007 | gene bg1314 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-1,4-glucan + H2O | beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose | Bacillus sp. (in: Bacteria) | 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose | - |
? | |
beta-1,3-1,4-glucan + H2O | from oat and barley | Bacillus sp. (in: Bacteria) | 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose | - |
? | |
beta-1,3-1,4-glucan + H2O | beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose | Bacillus sp. (in: Bacteria) SJ-10 | 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose | - |
? | |
beta-1,3-1,4-glucan + H2O | from oat and barley | Bacillus sp. (in: Bacteria) SJ-10 | 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose | - |
? | |
lichenan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
lichenan + H2O | - |
Bacillus sp. (in: Bacteria) SJ-10 | ? | - |
? | |
additional information | beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | thin layer product identification | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans | Bacillus sp. (in: Bacteria) SJ-10 | ? | - |
? | |
additional information | thin layer product identification | Bacillus sp. (in: Bacteria) SJ-10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26800, about, sequence calculation | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 90 | over 50% of maximal activity within this range, profile overview. The enzyme shows 75, 88, 90 and 80% activity at 20, 40, 60 and 80°C, respectively | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant His-tagged enzyme, pH 6.0, 30 min, completely stable | Bacillus sp. (in: Bacteria) |
65 | - |
purified recombinant His-tagged enzyme, pH 6.0, 30 min, loss of 50% activity | Bacillus sp. (in: Bacteria) |
70 | - |
purified recombinant His-tagged enzyme, pH 6.0, 30 min, inactivation | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | over 50% of maximal activity within this range, profile overview | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | purified recombinant His-tagged enzyme, 50°C, 30 min, completely stable | Bacillus sp. (in: Bacteria) |