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Literature summary for 3.2.1.73 extracted from

  • Kim, Y.R.; Kim, E.Y.; Lee, J.M.; Kim, J.K.; Kong, I.S.
    Characterisation of a novel Bacillus sp. SJ-10 beta-1,3-1,4-glucanase isolated from jeotgal, a traditional Korean fermented fish (2013), Bioprocess Biosyst. Eng., 36, 721-727.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bg1314, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is halotolerant Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26800
-
x * 26800, about, sequence calculation Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,3-1,4-glucan + H2O Bacillus sp. (in: Bacteria) beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose
-
?
beta-1,3-1,4-glucan + H2O Bacillus sp. (in: Bacteria) SJ-10 beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose
-
?
additional information Bacillus sp. (in: Bacteria) beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans ?
-
?
additional information Bacillus sp. (in: Bacteria) SJ-10 beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) I1W007 gene bg1314
-
Bacillus sp. (in: Bacteria) SJ-10 I1W007 gene bg1314
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-1,4-glucan + H2O beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose Bacillus sp. (in: Bacteria) 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose
-
?
beta-1,3-1,4-glucan + H2O from oat and barley Bacillus sp. (in: Bacteria) 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose
-
?
beta-1,3-1,4-glucan + H2O beta-1,3-1,4-glucanase can hydrolyse only the beta-1,4-glycosidic bond adjacent to beta-1,3-glycosidic bond in the mixed glycosidic linkages of beta-glucan, but cannot hydrolyse the beta-1,4-glycosidic bond in cellulose Bacillus sp. (in: Bacteria) SJ-10 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose
-
?
beta-1,3-1,4-glucan + H2O from oat and barley Bacillus sp. (in: Bacteria) SJ-10 3-O-cellobiosyl-D-glucose + 3-O-cellotriosyl-D-glucose
-
?
lichenan + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
lichenan + H2O
-
Bacillus sp. (in: Bacteria) SJ-10 ?
-
?
additional information beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans Bacillus sp. (in: Bacteria) ?
-
?
additional information thin layer product identification Bacillus sp. (in: Bacteria) ?
-
?
additional information beta-1,3-1,4-glucanase is an endo-beta-glucanase that cleaves the beta-1,4-linkage in the presence of alphabeta-1,3-linkage in mixed-linkage beta-1,3-1,4-glucans Bacillus sp. (in: Bacteria) SJ-10 ?
-
?
additional information thin layer product identification Bacillus sp. (in: Bacteria) SJ-10 ?
-
?

Subunits

Subunits Comment Organism
? x * 26800, about, sequence calculation Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 90 over 50% of maximal activity within this range, profile overview. The enzyme shows 75, 88, 90 and 80% activity at 20, 40, 60 and 80°C, respectively Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant His-tagged enzyme, pH 6.0, 30 min, completely stable Bacillus sp. (in: Bacteria)
65
-
purified recombinant His-tagged enzyme, pH 6.0, 30 min, loss of 50% activity Bacillus sp. (in: Bacteria)
70
-
purified recombinant His-tagged enzyme, pH 6.0, 30 min, inactivation Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
3 11 over 50% of maximal activity within this range, profile overview Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 purified recombinant His-tagged enzyme, 50°C, 30 min, completely stable Bacillus sp. (in: Bacteria)