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Literature summary for 3.2.1.73 extracted from

  • Hua, C.; Yan, Q.; Jiang, Z.; Li, Y.; Katrolia, P.
    High-level expression of a specific beta-1,3-1,4-glucanase from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris (2010), Appl. Microbiol. Biotechnol., 88, 509-518.
    View publication on PubMed
Search for more literature for 3.2.1.73

Application

Application Comment Organism
synthesis upon expression in Pichia pastoirs as active extracellular beta-1,3-1,4-glucanase, the recombinant protein is secreted predominantly into the medium and comprises up to 85% of the total extracellular proteins and reaches a protein concentration of 9.1 g/l with an activity of 55,300 U/ml in 5-l fermentor culture Paecilomyces sp. 'thermophila'

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Paecilomyces sp. 'thermophila'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31923
-
 x * 38500, SDS-PAGE, x * 31923, calculated Paecilomyces sp. 'thermophila'
38500
-
 x * 38500, SDS-PAGE, x * 31923, calculated Paecilomyces sp. 'thermophila'

Organism

Organism UniProt Comment Textmining
Paecilomyces sp. 'thermophila' E0XN39
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no N-glycosylation sites present in the protein, Endo H treatment does not result in a protein band shift Paecilomyces sp. 'thermophila'

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8208
-
 substrate lichenan, pH 7.0, 70°C Paecilomyces sp. 'thermophila'
11940
-
 substrate barley beta-glucan, pH 7.0, 70°C Paecilomyces sp. 'thermophila'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-1,4-glucan + H2O source of substrate: barley Paecilomyces sp. 'thermophila' ?
-
 ?
lichenan + H2O 68.8% of the activity with barley beta-glucan Paecilomyces sp. 'thermophila' ?
-
 ?
additional information strictly specific for beta-1,3-1,4-glucans. No substrates are: Avicel, carboxymethyl-cellulose, cellulose, filter paper, beta-1,3-glucan, laminarin, and birchwood xylan Paecilomyces sp. 'thermophila' ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38500, SDS-PAGE, x * 31923, calculated Paecilomyces sp. 'thermophila'

Synonyms

Synonyms Comment Organism
Lic16A
-
 Paecilomyces sp. 'thermophila'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Paecilomyces sp. 'thermophila'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, more than 90% of maximum activity Paecilomyces sp. 'thermophila'
70
-
 30 min, 65% of maximum activity Paecilomyces sp. 'thermophila'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Paecilomyces sp. 'thermophila'