Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trinitrobenzenesulfonic acid | - |
Thermomonospora sp. | |
5,5'-dithiobis 2-nitrobenzoic acid | - |
Thermomonospora sp. | |
o-phthaldehyde | - |
Thermomonospora sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64120 | - |
gel filtration | Thermomonospora sp. |
64240 | - |
calculated from amino acid sequence | Thermomonospora sp. |
64500 | - |
SDS-PAGE | Thermomonospora sp. |
66730 | - |
MALDI-TOF mass spectrometry | Thermomonospora sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomonospora sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sephadex A50 column chromatography and Sephacryl S-200 gel filtration | Thermomonospora sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.89 | - |
enzyme from culture filtrate, using xylan as a substrate, at 50°C, pH 7.0 | Thermomonospora sp. |
25 | - |
enzyme from culture filtrate, using lichenan as a substrate, at 50°C, pH 7.0 | Thermomonospora sp. |
598.6 | - |
after 31.6fold purification, using xylan as a substrate, at 50°C, pH 7.0 | Thermomonospora sp. |
792.2 | - |
after 31.6fold purification, using lichenan as a substrate, at 50°C, pH 7.0 | Thermomonospora sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lichenan + H2O | - |
Thermomonospora sp. | D-glucose + ? | - |
? | |
additional information | no activity towards avicel, laminarin, gum arabic, p-nitrophenyl glucoside, and p-nitrophenyl xylopyranoside | Thermomonospora sp. | ? | - |
? | |
xylan + H2O | - |
Thermomonospora sp. | xylose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1-3, 1-4 glucan 4-glucanohydrolase | - |
Thermomonospora sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermomonospora sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life of 5 min at 80°C | Thermomonospora sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermomonospora sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Thermomonospora sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomonospora sp. | isoelectric focusing | - |
4.27 |