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Literature summary for 3.2.1.73 extracted from
- Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris (2007), Appl. Microbiol. Biotechnol., 74, 1074-1083.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| beta-mercaptoethanol | 0.1% | Bacillus licheniformis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM | Bacillus licheniformis | |
| additional information | no significant effects are found for Ca2+, Mg2+, glycerol, D-sorbitol, boric acid, and EDTA | Bacillus licheniformis | |
| Zn2+ | 10 mM | Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates at 10 mM | Bacillus licheniformis | |
| Fe2+ | activates at 10 mM | Bacillus licheniformis | |
| Mn2+ | activates at 10 mM | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis EGW039 / CGMCC 0635 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1280 | - |
recombinant enzyme, using barley beta-glucan as a substrate, at 50°C and pH 6.0 | Bacillus licheniformis |
| 1335 | - |
recombinant enzyme, using lichenan as a substrate, at 50°C and pH 6.0 | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| barley beta-glucan + H2O | - |
Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
| lichenan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| lichenan + H2O | - |
Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
| additional information | no activity on cellulose | Bacillus licheniformis | ? | - |
? | |
| additional information | no activity on cellulose | Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant enzyme | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | after incubation at 50, 60, and 70°C for 30 min, the residual activity is 95, 60, and 0%, respectively | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
retains approximately 80% of original activity after treatment at pH 3.0 for 12 h | Bacillus licheniformis |
| 4 | 6 | the recombinant enzyme is stable between pH 4.0 and 6.0, having 85% of the original activity | Bacillus licheniformis |
| 8 | - |
retains approximately 80% of original activity after treatment at pH 8.0 for 12 h | Bacillus licheniformis |
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