Any feedback?
Literature summary for 3.2.1.73 extracted from
- Cloning of beta-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3) (2006), Appl. Microbiol. Biotechnol., 72, 705-712.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21 (DE3) | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
recombinant enzyme with a six-His tag at the N terminus, SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis EGW039 / CGMCC 0635 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA His Bind resin column chromatography | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 283.8 | - |
recombinant enzyme from crude extract, using barley beta-glucan as a substrate | Bacillus licheniformis |
| 1906 | - |
recombinant enzyme, using lichenan as a substrate | Bacillus licheniformis |
| 2479 | - |
recombinant enzyme after 8.74fold purification, using barley beta-glucan as a substrate | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Bacillus licheniformis | ? | - |
? |  |
| barley beta-glucan + H2O | - |
Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
| lichenan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| lichenan + H2O | - |
Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
| additional information | activity against 1% carboxymethylcellulose is not observed | Bacillus licheniformis | ? | - |
? | |
| additional information | activity against 1% carboxymethylcellulose is not observed | Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
recombinant enzyme | Bacillus licheniformis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
