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Literature summary for 3.2.1.73 extracted from

  • Stewart, R.J.; Varghese, J.N.; Garrett, T.P.J.; Hoj, P.B.; Fincher, G.B.
    Mutant barley (1-3,1-4)-beta-glucan endohydrolases with enhanced thermostability (2001), Protein Eng., 14, 245-253.
    View publication on PubMed
Search for more literature for 3.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
A79P decrease in thermal stability Hordeum vulgare
F85Y decrease in thermal stability Hordeum vulgare
G44R decrease in thermal stability Hordeum vulgare
H300P increase in thermal stability Hordeum vulgare
K23R decrease in thermal stability Hordeum vulgare
M298K thermal stabiltiy similar to wild type Hordeum vulgare
N290H slight increase in thermal stability Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
 x * 33500, SDS-PAGE, recombinant enzyme with His-tag Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.5
-
 37°C, pH 5.0 Hordeum vulgare

Storage Stability

Storage Stability Organism
4°C, 50 mM sodium phosphate, pH 7.8, 300 mM sodium chloride, stable for several weeks Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1-3,1-4)-beta-D-glucan + H2O i.e. barley (1-3,1-4)-beta-glucan Hordeum vulgare ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33500, SDS-PAGE, recombinant enzyme with His-tag Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44.5
-
 15 min, 50% of activity, mutant G44R Hordeum vulgare
46
-
 15 min, 50% of activity, mutant K23R Hordeum vulgare
46.1
-
 15 min, 50% of activity, mutant A79P Hordeum vulgare
46.2
-
 15 min, 50% of activity, mutant F85Y Hordeum vulgare
47.5
-
 15 min, 50% of activity, wild type Hordeum vulgare
47.9
-
 15 min, 50% of activity, mutant M298K, mutant T17D Hordeum vulgare
48.2
-
 15 min, 50% of activity, mutant N290H Hordeum vulgare
51.2
-
 15 min, 50% of activity, mutant H300P Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 wild type, mutant H300P Hordeum vulgare