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Literature summary for 3.2.1.73 extracted from

  • Cheng, H.L.; Tsai, L.C.; Lin, S.S.; Yuan, H.S.; Yang, N.S.; Lee, S.H.; Shyur, L.F.
    Mutagenesis of Trp(54) and Trp(203) residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalytic activities of the enzyme (2002), Biochemistry, 41, 8759-8766.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
W105F significant decrease in thermostability Fibrobacter succinogenes
W105H significant decrease in thermostability Fibrobacter succinogenes
W141F 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
W141H 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
W148F decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
W165F after incubation at pH 3.0, 1 h, 3-7-fold higher activity than wild type Fibrobacter succinogenes
W165H significant decrease in thermostability Fibrobacter succinogenes
W186F increase in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
W198F significant decrease in thermostability Fibrobacter succinogenes
W203F increase in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
W203R 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, after incubation at pH 3.0, 1 h, 3-7-fold higher activity than wild type, no significant change in thermal stability Fibrobacter succinogenes
W54F decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
W54Y decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes

Organism

Organism UniProt Comment Textmining
Fibrobacter succinogenes
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15 19 pH 6.0, mutant W105H Fibrobacter succinogenes
29
-
pH 6.0, mutant W54F Fibrobacter succinogenes
37
-
pH 6.0, mutant W54Y Fibrobacter succinogenes
69
-
pH 6.0, mutant W141H Fibrobacter succinogenes
70
-
pH 6.0, mutant W203R Fibrobacter succinogenes
419
-
pH 6.0, mutant W141F Fibrobacter succinogenes
480
-
pH 6.0, mutant W148F Fibrobacter succinogenes
916
-
pH 6.0, mutant W112F Fibrobacter succinogenes
1572
-
pH 6.0, mutant W165H Fibrobacter succinogenes
1621
-
pH 6.0, mutant W105F Fibrobacter succinogenes
1755
-
pH 6.0, mutant W198F Fibrobacter succinogenes
1791
-
pH 6.0, mutant W165F Fibrobacter succinogenes
2065
-
pH 6.0, wild type Fibrobacter succinogenes
2941
-
pH 6.0, mutant W186F Fibrobacter succinogenes
8726
-
pH 6.0, mutant W203F Fibrobacter succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenan + H2O
-
Fibrobacter succinogenes ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
unstable above Fibrobacter succinogenes

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Fibrobacter succinogenes