Protein Variants | Comment | Organism |
---|---|---|
W105F | significant decrease in thermostability | Fibrobacter succinogenes |
W105H | significant decrease in thermostability | Fibrobacter succinogenes |
W141F | 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
W141H | 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
W148F | decrease in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
W165F | after incubation at pH 3.0, 1 h, 3-7-fold higher activity than wild type | Fibrobacter succinogenes |
W165H | significant decrease in thermostability | Fibrobacter succinogenes |
W186F | increase in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
W198F | significant decrease in thermostability | Fibrobacter succinogenes |
W203F | increase in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
W203R | 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, after incubation at pH 3.0, 1 h, 3-7-fold higher activity than wild type, no significant change in thermal stability | Fibrobacter succinogenes |
W54F | decrease in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
W54Y | decrease in kcat-value, no significant change in thermal stability | Fibrobacter succinogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fibrobacter succinogenes | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | 19 | pH 6.0, mutant W105H | Fibrobacter succinogenes |
29 | - |
pH 6.0, mutant W54F | Fibrobacter succinogenes |
37 | - |
pH 6.0, mutant W54Y | Fibrobacter succinogenes |
69 | - |
pH 6.0, mutant W141H | Fibrobacter succinogenes |
70 | - |
pH 6.0, mutant W203R | Fibrobacter succinogenes |
419 | - |
pH 6.0, mutant W141F | Fibrobacter succinogenes |
480 | - |
pH 6.0, mutant W148F | Fibrobacter succinogenes |
916 | - |
pH 6.0, mutant W112F | Fibrobacter succinogenes |
1572 | - |
pH 6.0, mutant W165H | Fibrobacter succinogenes |
1621 | - |
pH 6.0, mutant W105F | Fibrobacter succinogenes |
1755 | - |
pH 6.0, mutant W198F | Fibrobacter succinogenes |
1791 | - |
pH 6.0, mutant W165F | Fibrobacter succinogenes |
2065 | - |
pH 6.0, wild type | Fibrobacter succinogenes |
2941 | - |
pH 6.0, mutant W186F | Fibrobacter succinogenes |
8726 | - |
pH 6.0, mutant W203F | Fibrobacter succinogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lichenan + H2O | - |
Fibrobacter succinogenes | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
unstable above | Fibrobacter succinogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Fibrobacter succinogenes |