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Literature summary for 3.2.1.73 extracted from

  • Erfle, J.D.; Teather, R.M.; Wood, P.J.; Irvin, J.E.
    Purification and properties of a 1,3-1,4-beta-D-glucanase (lichenase, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, EC 3.2.1.73) from Bacteroides succinogenes cloned in Escherichia coli (1988), Biochem. J., 255, 833-841.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fibrobacter succinogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Fibrobacter succinogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35200
-
 1 * 35200, SDS-PAGE Fibrobacter succinogenes
37200
-
 gel filtration Fibrobacter succinogenes

Organism

Organism UniProt Comment Textmining
Fibrobacter succinogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fibrobacter succinogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1372
-
-
 Fibrobacter succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucan + H2O beta-D-glucan from oat Fibrobacter succinogenes additional information
-
 ?
lichenin + H2O
-
 Fibrobacter succinogenes additional information
-
 ?
additional information no hydrolysis of carboxymethylpachyman Fibrobacter succinogenes ?
-
 ?
additional information no hydrolysis of starch Fibrobacter succinogenes ?
-
 ?
additional information no hydrolysis of xylan Fibrobacter succinogenes ?
-
 ?
additional information no hydrolysis of laminarin Fibrobacter succinogenes ?
-
 ?
additional information no hydrolysis of carboxymethylcellulose Fibrobacter succinogenes ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35200, SDS-PAGE Fibrobacter succinogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 lichenin or oat beta-D-glucan as substrate Fibrobacter succinogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C, about 30% of maximal activity with oat beta-D-glucan, about 45% of maximal activity with lichenin, 60°C: about 20% of maximal activity with lichenin and oat beta-D-glucan as substrate Fibrobacter succinogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, 50% loss of activity, crude enzyme extract Fibrobacter succinogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6.3
-
 Fibrobacter succinogenes