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Literature summary for 3.2.1.70 extracted from

  • Delgado, S.; Florez, A.B.; Guadamuro, L.; Mayo, B.
    Genetic and biochemical characterization of an oligo-alpha-1,6-glucosidase from Lactobacillus plantarum (2017), Int. J. Food Microbiol., 246, 32-39 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3) cells Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.22
-
isomaltose at pH 5.5 and 37°C Lactiplantibacillus plantarum
244.52
-
isomaltulose at pH 5.5 and 37°C Lactiplantibacillus plantarum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Lactiplantibacillus plantarum 5737
-

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum LL441
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O strong hydrolytic activity Lactiplantibacillus plantarum 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O strong hydrolytic activity Lactiplantibacillus plantarum LL441 4-nitrophenol + D-glucopyranose
-
?
isomaltose + H2O 54.06% hydrolysis Lactiplantibacillus plantarum 2 D-glucose
-
?
isomaltose + H2O 54.06% hydrolysis Lactiplantibacillus plantarum LL441 2 D-glucose
-
?
isomaltulose + H2O 37.3% hydrolysis Lactiplantibacillus plantarum D-glucose
-
?
isomaltulose + H2O 37.3% hydrolysis Lactiplantibacillus plantarum LL441 D-glucose
-
?
additional information the enzyme has no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-manopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-fucopyranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl alpha-D-maltohexaoside, isomaltrotriose, melibiose, panose and raffinose, trehalose, sucrose, turanose or maltose. The enzyme shows no transglycosylation activity Lactiplantibacillus plantarum ?
-
?
additional information the enzyme has no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-manopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-fucopyranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl alpha-D-maltohexaoside, isomaltrotriose, melibiose, panose and raffinose, trehalose, sucrose, turanose or maltose. The enzyme shows no transglycosylation activity Lactiplantibacillus plantarum LL441 ?
-
?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Lactiplantibacillus plantarum
? x * 66310, calculated from amino acid sequence Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
MalL
-
Lactiplantibacillus plantarum
oligo-alpha-1,6-glucosidase
-
Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 the activity of the enzyme declines sharply before 20°C and after 40°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 the enzyme shows high specific activity between pH 5.0 and 6.0, with no clear maximum Lactiplantibacillus plantarum