Cloned (Comment) | Organism |
---|---|
overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3) cells | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.22 | - |
isomaltose | at pH 5.5 and 37°C | Lactiplantibacillus plantarum | |
244.52 | - |
isomaltulose | at pH 5.5 and 37°C | Lactiplantibacillus plantarum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Lactiplantibacillus plantarum | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Lactiplantibacillus plantarum LL441 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | strong hydrolytic activity | Lactiplantibacillus plantarum | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | strong hydrolytic activity | Lactiplantibacillus plantarum LL441 | 4-nitrophenol + D-glucopyranose | - |
? | |
isomaltose + H2O | 54.06% hydrolysis | Lactiplantibacillus plantarum | 2 D-glucose | - |
? | |
isomaltose + H2O | 54.06% hydrolysis | Lactiplantibacillus plantarum LL441 | 2 D-glucose | - |
? | |
isomaltulose + H2O | 37.3% hydrolysis | Lactiplantibacillus plantarum | D-glucose | - |
? | |
isomaltulose + H2O | 37.3% hydrolysis | Lactiplantibacillus plantarum LL441 | D-glucose | - |
? | |
additional information | the enzyme has no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-manopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-fucopyranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl alpha-D-maltohexaoside, isomaltrotriose, melibiose, panose and raffinose, trehalose, sucrose, turanose or maltose. The enzyme shows no transglycosylation activity | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | the enzyme has no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-manopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-fucopyranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl alpha-D-maltohexaoside, isomaltrotriose, melibiose, panose and raffinose, trehalose, sucrose, turanose or maltose. The enzyme shows no transglycosylation activity | Lactiplantibacillus plantarum LL441 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, SDS-PAGE | Lactiplantibacillus plantarum |
? | x * 66310, calculated from amino acid sequence | Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
MalL | - |
Lactiplantibacillus plantarum |
oligo-alpha-1,6-glucosidase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactiplantibacillus plantarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the activity of the enzyme declines sharply before 20°C and after 40°C | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | the enzyme shows high specific activity between pH 5.0 and 6.0, with no clear maximum | Lactiplantibacillus plantarum |