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Literature summary for 3.2.1.7 extracted from

  • Chen, H.; Chen, X.; Li, Y.; Wang, J.; Jin, Z.; Xu, X.; Zhao, J.; Chen, T.; Xie, Z.
    Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06 (2009), Food Chem., 115, 1206-1212.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition, both isoforms Endo-I and Endo-II Aspergillus ficuum
Al3+ strong inhibition, both isoforms Endo-I and Endo-II Aspergillus ficuum
Fe2+ strong inhibition, both isoforms Endo-I and Endo-II Aspergillus ficuum
additional information not inhibitory: K+, Ca2+, Li+, EDTA, urea Aspergillus ficuum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
inulin Km value is 14.8 mg per ml for isoform Endo-I, and 25.6 mg per ml for isoform Endo-II, pH 5.0, 50°C Aspergillus ficuum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus ficuum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 34000, isoform Endo-I, x * 31000, isoform Endo-II, SDS-PAGE Aspergillus ficuum
34000
-
x * 34000, isoform Endo-I, x * 31000, isoform Endo-II, SDS-PAGE Aspergillus ficuum

Organism

Organism UniProt Comment Textmining
Aspergillus ficuum
-
-
-
Aspergillus ficuum JNSP5-06
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
Aspergillus ficuum ?
-
?
inulin + H2O
-
Aspergillus ficuum JNSP5-06 ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, isoform Endo-I, x * 31000, isoform Endo-II, SDS-PAGE Aspergillus ficuum

Synonyms

Synonyms Comment Organism
Endo-I
-
Aspergillus ficuum
Endo-II
-
Aspergillus ficuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
both isoforms Endo-I and Endo-II Aspergillus ficuum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable below, both isoforms Endo-I and Endo-II Aspergillus ficuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
both isoforms Endo-I and Endo-II Aspergillus ficuum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 both isoforms Endo-I and Endo-II Aspergillus ficuum