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Literature summary for 3.2.1.7 extracted from

  • Skowronek, M.; Fiedurek, J.
    Purification and properties of extracellular endoinulinase from Aspergillus niger 20 OSM (2006), Food Technol. Biotechnol., 44, 53-58.
No PubMed abstract available
Search for more literature for 3.2.1.7

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, strong inhibition Aspergillus niger
Co2+ 1 mM, strong inhibition Aspergillus niger
EDTA 1 mM, 83% inhibition Aspergillus niger
Fe3+ 1 mM, strong inhibition Aspergillus niger
Hg2+ 1 mM, complete inactivation Aspergillus niger
Mn2+ 1 mM, complete inactivation Aspergillus niger
Ni2+ 1 mM, strong inhibition Aspergillus niger
p-chloromercuribenzoate
-
 Aspergillus niger
Zn2+ 1 mM, strong inhibition Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus niger
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 gel filtration Aspergillus niger
69000
-
 1 * 69000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 20 OSM
-
Aspergillus niger 20 OSM
-
 20 OSM
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content is about 44.5% Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1158
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O during the hydrolysis process, at first longer saccharide chains appear, which are subsequently cut into shorter fragments Aspergillus niger ?
-
 ?
inulin + H2O during the hydrolysis process, at first longer saccharide chains appear, which are subsequently cut into shorter fragments Aspergillus niger 20 OSM ?
-
 ?
additional information purified inulinase shows no detectable activity toward sucrose and little activity toward raffinose (0.8% of relative enzyme activity on inulin) Aspergillus niger ?
-
 ?
additional information purified inulinase shows no detectable activity toward sucrose and little activity toward raffinose (0.8% of relative enzyme activity on inulin) Aspergillus niger 20 OSM ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 69000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
endoinulinase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable below Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7 stable Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger chromatofocusing
-
 3.85