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Literature summary for 3.2.1.68 extracted from

  • Kubo, A.; Colleoni, C.; Dinges, J.R.; Lin, Q.; Lappe, R.R.; Rivenbark, J.G.; Meyer, A.J.; Ball, S.G.; James, M.G.; Hennen-Bierwagen, T.A.; Myers, A.M.
    Functions of heteromeric and homomeric isoamylase-type starch-debranching enzymes in developing maize endosperm (2010), Plant Physiol., 153, 956-969.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant GST-ISA2 fusion protein is expressed in Escherichia coli Rosetta DE3-pLysS cells Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
wild type endosperm contains three high molecular mass ISA complexes resolved by gel filtration and native-PAGE. Two complexes of approximately 400 kDa contain both isoform ISA1 and isoform ISA2, and an approximately 300 kDa complex contains isoform ISA1 but not isoform ISA2 Zea mays
400000
-
wild type endosperm contains three high molecular mass ISA complexes resolved by gel filtration and native-PAGE. Two complexes of approximately 400 kDa contain both isoform ISA1 and isoform ISA2, and an approximately 300 kDa complex contains isoform ISA1 but not isoform ISA2 Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycogen + H2O Zea mays
-
maltose + maltooligosaccharides
-
?

Organism

Organism UniProt Comment Textmining
Zea mays Q84UE6 isoform ISA2
-

Purification (Commentary)

Purification (Comment) Organism
HiTrapQ column chromatography and Superdex 200 gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + H2O
-
Zea mays maltose + maltooligosaccharides
-
?

Synonyms

Synonyms Comment Organism
ISA
-
Zea mays
isoamylase-type starch-debranching enzyme
-
Zea mays

Expression

Organism Comment Expression
Zea mays the isoform ISA1 transcript level is low during starch degradation down
Zea mays isoform ISA2 transcript is relatively abundant during periods of either starch biosynthesis or catabolism additional information
Zea mays the isoform ISA1 transcript level is elevated in tissues where starch is synthesized up

General Information

General Information Comment Organism
physiological function the isoamylase ISA1/ISA2 heteromeric complexes II and III are not required in maize endosperm for normal starch content and structure, and the ISA1 homomeric complex is sufficient for most ISA functions. ISA1 is required for the accumulation of ISA2, which is regulated posttranscriptionally, while the absence of ISA2 in isa2-339 mutants has no effect on the accumulation of ISA1 mRNA or protein Zea mays