Cloned (Comment) | Organism |
---|---|
recombinant GST-ISA2 fusion protein is expressed in Escherichia coli Rosetta DE3-pLysS cells | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
wild type endosperm contains three high molecular mass ISA complexes resolved by gel filtration and native-PAGE. Two complexes of approximately 400 kDa contain both isoform ISA1 and isoform ISA2, and an approximately 300 kDa complex contains isoform ISA1 but not isoform ISA2 | Zea mays |
400000 | - |
wild type endosperm contains three high molecular mass ISA complexes resolved by gel filtration and native-PAGE. Two complexes of approximately 400 kDa contain both isoform ISA1 and isoform ISA2, and an approximately 300 kDa complex contains isoform ISA1 but not isoform ISA2 | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | Zea mays | - |
maltose + maltooligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q84UE6 | isoform ISA2 | - |
Purification (Comment) | Organism |
---|---|
HiTrapQ column chromatography and Superdex 200 gel filtration | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | - |
Zea mays | maltose + maltooligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ISA | - |
Zea mays |
isoamylase-type starch-debranching enzyme | - |
Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | the isoform ISA1 transcript level is low during starch degradation | down |
Zea mays | isoform ISA2 transcript is relatively abundant during periods of either starch biosynthesis or catabolism | additional information |
Zea mays | the isoform ISA1 transcript level is elevated in tissues where starch is synthesized | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the isoamylase ISA1/ISA2 heteromeric complexes II and III are not required in maize endosperm for normal starch content and structure, and the ISA1 homomeric complex is sufficient for most ISA functions. ISA1 is required for the accumulation of ISA2, which is regulated posttranscriptionally, while the absence of ISA2 in isa2-339 mutants has no effect on the accumulation of ISA1 mRNA or protein | Zea mays |