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Literature summary for 3.2.1.68 extracted from

  • Utsumi, Y.; Nakamura, Y.
    Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm (2006), Planta, 225, 75-87.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of OsISA2 in Escherichia coli Oryza sativa
expression of OsISA2in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
amylose-extender amylopectin hetero-oligomer Oryza sativa
0.00004
-
amylose-extender amylopectin homo-oligomer Oryza sativa
0.00012
-
amylopectin hetero-oligomer Oryza sativa
0.00018
-
beta-limit dextrin hetero-oligomer Oryza sativa
0.00021
-
beta-limit dextrin homo-oligomer Oryza sativa
0.00025
-
amylopectin homo-oligomer Oryza sativa
0.00062
-
oyster glycogen hetero-oligomer Oryza sativa
0.00067
-
oyster glycogen homo-oligomer Oryza sativa
0.0054
-
phytoglycogen hetero-oligomer Oryza sativa
0.0065
-
phytoglycogen homo-oligomer Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration Oryza sativa
420000
-
SDS-PAGE, homooligomer, five OsISA1 Oryza sativa
510000
-
SDS-PAGE, heterooligomer, hetero-hexamer composed of five OsISA1 and one OsISA2 Oryza sativa
830000
-
homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
L. cv. Nipponbare
-
Oryza sativa O80403 L. cv. Nipponbare
-

Purification (Commentary)

Purification (Comment) Organism
wild type enzyme by HitrapQ HP and TSKgel G3000SWXL column chromatography, recombinant OsISA2 by HitrapQ HP column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Oryza sativa maltose + maltooligosaccharides
-
?
amylose-extender amylopectin + H2O
-
Oryza sativa maltose + maltooligosaccharides
-
?
beta limit dextrin + H2O from maize Oryza sativa maltose + maltooligosaccharides
-
?
oyster glycogen + H2O
-
Oryza sativa ?
-
?
oyster gylcogen + H2O
-
Oryza sativa maltose + maltooligosaccharides
-
?
phytoglycogen + H2O
-
Oryza sativa maltose + maltooligosaccharides
-
?
starch + H2O
-
Oryza sativa maltose + maltooligosaccharides
-
?

Subunits

Subunits Comment Organism
oligomer heterooligomer OsISA1-OsISA2 6*85000, HPLC gel filtration Oryza sativa
oligomer homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration Oryza sativa

Synonyms

Synonyms Comment Organism
glycogen alpha-1,6-glucanohydrolase
-
Oryza sativa
isoamylase
-
Oryza sativa
OsISA1
-
Oryza sativa
OsISA2
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
homo-oligomer Oryza sativa
40
-
hetero-oligomer Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
homooligomer, complete loss of activity Oryza sativa
40 50 hetero-oligomer, loss of 50% activity Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Oryza sativa