Application | Comment | Organism |
---|---|---|
detergent | effective additive in dishwashing and laundry detergents | Bacillus sp. (in: Bacteria) |
nutrition | - |
Bacillus sp. (in: Bacteria) |
nutrition | - |
Pseudomonas amyloderamosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 52% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | - |
Pseudomonas amyloderamosa | |
N-bromosuccinimide | 0.01 mg abolishes enzyme activity almost completely | Bacillus sp. (in: Bacteria) | |
N-bromosuccinimide | - |
Pseudomonas amyloderamosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | potatoamylopectin 0.59 mg/ml-1, oyster glycogen 0.45 mg/ml-1 | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE, gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
KSM.3309, gram-positive, isolated from soil | - |
Pseudomonas amyloderamosa | - |
SMP1 | - |
Pseudomonas amyloderamosa SMP1 | - |
SMP1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
469 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | specific cleavage of alpha 1,6-glucosidic inter-chain linkages producing essentially linear chains | Bacillus sp. (in: Bacteria) | maltose + maltooligosaccharides | - |
? | |
amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Bacillus sp. (in: Bacteria) | maltose + maltooligosaccharides | - |
? | |
amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Pseudomonas amyloderamosa SMP1 | maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | cleaves the branching points completely in vitro | Bacillus sp. (in: Bacteria) | maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | or the beta-limit dextrins | Bacillus sp. (in: Bacteria) | maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | or the beta-limit dextrins | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | cleaves the branching points completely in vitro | Pseudomonas amyloderamosa SMP1 | maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | or the beta-limit dextrins | Pseudomonas amyloderamosa SMP1 | maltose + maltooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pseudomonas amyloderamosa |
monomer | purified enzyme has monomeric structure | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
alkaline isoamylase | alkaliphilic Bacillus sp KSM-3309, isolated from soil | Bacillus sp. (in: Bacteria) |
glycogen 6-glucanohydrolase | - |
Bacillus sp. (in: Bacteria) |
glycogen 6-glucanohydrolase | - |
Pseudomonas amyloderamosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to 40°C, at temperature below 30°C and above 65°C enzyme is almost inactive | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11.5 | more than 50% of the original activity detectable between pH 6.6-10.5 | Bacillus sp. (in: Bacteria) |