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Literature summary for 3.2.1.68 extracted from
- Extracellular isoamylase produced by the yeast Lipomyces kononenkoae (1982), Appl. Environ. Microbiol., 44, 1253-1257.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | production of high-maltose syrups and highly purified maltose by a combination of debranching enzymes | Lipomyces kononenkoae |
General Stability
| General Stability | Organism |
|---|---|
| very unstable, even at low temperatures | Lipomyces kononenkoae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Lipomyces kononenkoae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration, 0.5-m Bio-Gel A | Lipomyces kononenkoae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Lipomyces kononenkoae | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lipomyces kononenkoae | - |
yeast, strain IGC 4051 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lipomyces kononenkoae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Lipomyces kononenkoae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Lipomyces kononenkoae | maltose + maltooligosaccharides | - |
? | |
| D-glucose + H2O | 59% of the activity with dextrin as substrate | Lipomyces kononenkoae | ? | - |
? | |
| dextrin + H2O | enzyme activity 100% | Lipomyces kononenkoae | ? | - |
? | |
| glycogen + H2O | cleaves the branching points completely in vitro | Lipomyces kononenkoae | maltose + maltooligosaccharides | - |
? | |
| glycogen + H2O | or the beta-limit dextrins | Lipomyces kononenkoae | maltose + maltooligosaccharides | - |
? | |
| maltotriose + H2O | activity 28% | Lipomyces kononenkoae | ? | - |
? | |
| panose + H2O | acts very slowly, small amounts of product formed after 24 h | Lipomyces kononenkoae | D-glucose + maltose | - |
? | |
| saccharose + H2O | activity 67% | Lipomyces kononenkoae | ? | - |
? | |
| starch + H2O | - |
Lipomyces kononenkoae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen 6-glucanohydrolase | - |
Lipomyces kononenkoae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
starch | Lipomyces kononenkoae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
starch | Lipomyces kononenkoae |
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Release 2023.1 (January 2023)
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