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Literature summary for 3.2.1.67 extracted from
- Isolation and heterologous expression of a polygalacturonase produced by Fusarium oxysporum f. sp. cubense race 1 and 4 (2015), Int. J. Mol. Sci., 16, 7595-7607 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain SMD1168 | Fusarium oxysporum f. sp. cubense |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium oxysporum f. sp. cubense | A0A0E3U204 | race 1 | - |
| Fusarium oxysporum f. sp. cubense | A0A0E3Y582 | race 4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose column chromatography and Sephacryl S-100gel filtration | Fusarium oxysporum f. sp. cubense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| banana stem tissue + H2O | maceration activity | Fusarium oxysporum f. sp. cubense | ? | - |
? |  |
| citrus pectin + H2O | - |
Fusarium oxysporum f. sp. cubense | galacturonic acid | - |
? | |
| polygalacturonate + H2O | - |
Fusarium oxysporum f. sp. cubense | galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Fusarium oxysporum f. sp. cubense |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exo-PG | - |
Fusarium oxysporum f. sp. cubense |
| PGC3 | - |
Fusarium oxysporum f. sp. cubense |
| polygalacturonanase | - |
Fusarium oxysporum f. sp. cubense |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Fusarium oxysporum f. sp. cubense |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | the enzyme retains more than 50% activity at 10-50°C | Fusarium oxysporum f. sp. cubense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | the enzyme retains more than 70% activity after 24 h incubation at 10-40 | Fusarium oxysporum f. sp. cubense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Fusarium oxysporum f. sp. cubense |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | the enzyme retains more than 40% activity at pH 3.0-7.0 | Fusarium oxysporum f. sp. cubense |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | the enzyme retains more than 70% activity after 24 h incubation at pH 3.0-6.5 | Fusarium oxysporum f. sp. cubense |
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Release 2023.1 (January 2023)
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