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Literature summary for 3.2.1.67 extracted from
- Improvement of activity, thermo-stability and fruit juice clarification characteristics of fungal exo-polygalacturonase (2017), Int. J. Biol. Macromol., 95, 974-984 .
General Stability
| General Stability | Organism |
|---|---|
| the affinity of enzyme towards its substrate is reduced after immobilization on sodium-alginate matrix and Vmax of covalently immobilized enzyme decrease to 66.7% while the Vmax value of adsorbed enzyme increase up to 150% as compared to free counterpart | Penicillium chrysogenum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Penicillium chrysogenum | - |
galacturonic acid + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Penicillium chrysogenum | galacturonic acid + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exo-PG | - |
Penicillium chrysogenum |
| exo-polygalacturonanase | - |
Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
free enzyme | Penicillium chrysogenum |
| 55 | - |
immobilized enzyme | Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | at 60°C, the sodium-alginate-matrix-immobilized enzyme retains more than 90% of its activity after 60 min of heating, while free enzyme does not show any activity at the same temperature. At 90°C, the half-life value of covalently immobilized enzyme is recorded 138.6 min while adsorbed enzyme shows a half-life of 115.5 min, whereas, the free enzyme displays 2.39 min half-life at 80°C | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
immobilized enzyme | Penicillium chrysogenum |
| 6 | - |
free enzyme | Penicillium chrysogenum |
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Release 2023.1 (January 2023)
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