Application | Comment | Organism |
---|---|---|
synthesis | mutant strain M3 can be used for production of exopolygalacturonase | Aspergillus sojae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant strain M3 with increased enzyme activity | Aspergillus sojae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-galacturonide]n + H2O | Aspergillus sojae | - |
[(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | Aspergillus sojae ATCC 20235 | - |
[(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sojae | - |
- |
- |
Aspergillus sojae ATCC 20235 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | solid-state fermentation | Aspergillus sojae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
with pectin as substrate: wild-type strain 97.8 U/ml, mutant strain M3 105.0 U/ml, mutant strain GH56 105.2 U/ml, and mutant strain Guserbiot 2.230 95.1 U/ml. Mutant strain M3 yields of enzyme activity is 1111.7 U/g on sugar beet and 142.1 U/g on apple pomace, while the wild-type strain gives 718.1 U/g and 240.0 U/g for sugar beet and apple pomace, respectively. Comprisons with commercial pectinases, overview | Aspergillus sojae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Aspergillus sojae | ? | - |
? | |
pectin + H2O | - |
Aspergillus sojae ATCC 20235 | ? | - |
? | |
polygalacturonic acid + H2O | - |
Aspergillus sojae | ? | - |
? | |
polygalacturonic acid + H2O | - |
Aspergillus sojae ATCC 20235 | ? | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | - |
Aspergillus sojae | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | - |
Aspergillus sojae ATCC 20235 | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo-PG | - |
Aspergillus sojae |
exo-polygalacturonase | - |
Aspergillus sojae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus sojae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
assay at | Aspergillus sojae |