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Literature summary for 3.2.1.67 extracted from

  • Dong, Z.; Wang, Z.
    Isolation and characterization of an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 1 and race 4 (2011), BMC Biochem., 12, 51.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.67

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strains of SMD1168 Fusarium oxysporum f. sp. cubense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51700
-
 calculated from amino acid sequence and determined by electrospray ionization mass spectrometry Fusarium oxysporum f. sp. cubense
63000
-
 1 * 63000, SDS-PAGE Fusarium oxysporum f. sp. cubense

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum f. sp. cubense D1MI97 race 4
-
Fusarium oxysporum f. sp. cubense D2KKQ2 race 1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Fusarium oxysporum f. sp. cubense

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, Sepharose FF CM Hitrap column chromatography, and Sephacryl S-100 gel filtration Fusarium oxysporum f. sp. cubense
ultrfiltration, Sepharose FF CM Hitrap column chromatography, and Sephacryl S-100 gel filtration Fusarium oxysporum f. sp. cubense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonate + H2O
-
 Fusarium oxysporum f. sp. cubense D-galacturonic acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 63000, SDS-PAGE Fusarium oxysporum f. sp. cubense

Synonyms

Synonyms Comment Organism
exoPG
-
 Fusarium oxysporum f. sp. cubense
exopolygalacturonase
-
 Fusarium oxysporum f. sp. cubense
PGC2
-
 Fusarium oxysporum f. sp. cubense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Fusarium oxysporum f. sp. cubense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 recombinant enzyme retains more than 50% activity at 10-50°C in 100 mM potassium phosphate buffer, pH 5.0, after 2 h incubation Fusarium oxysporum f. sp. cubense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Fusarium oxysporum f. sp. cubense

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 recombinant enzyme retains more than 70% activity at pH 3.0-6.0 after 24 h at 4°C Fusarium oxysporum f. sp. cubense

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium oxysporum f. sp. cubense calculated from amino acid sequence
-
 8.3

General Information

General Information Comment Organism
physiological function recombinant PGC2 protein can induce tissue maceration and necrosis in banana plants Fusarium oxysporum f. sp. cubense