General Stability | Organism |
---|---|
stability studies of the enzyme at different pH values after 24 h of incubation at room temperature give the highest percentage residual activities as follows: 23.01% at pH 4.5 in acetate, 28.32% at pH 7.0 in phosphate, and 22.13% at pH 7.5 in Tris-HCl | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE | Aspergillus niger |
40000 | - |
1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger SA6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75 gel filtration | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.99 | - |
crude enzyme, at pH 4.5 and 40°C | Aspergillus niger |
246 | - |
after 9.1fold purification, at pH 4.5 and 40°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citric pectin + H2O | - |
Aspergillus niger | D-galacturonic acid + ? | - |
? | |
citric pectin + H2O | - |
Aspergillus niger SA6 | D-galacturonic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
exoPG | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | about 55% activity at 30-40°C, about 45% activity at 50°C, about 25% activity at 60°C, about 15% activity at 70°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | - |
Aspergillus niger |