General Stability | Organism |
---|---|
calcium alginate-immobilized polygalacturonase exhibits more stability to changes in pH than the temperature. The activity of the immobilized polygalacturonase reduces to 34.56% and 14.81% of the initial activity in the second and third catalytic cycles, respectively | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 40000 + 1 * 35000, endo- and exo-polygalacturonase, SDS-PAGE | Aspergillus niger |
40000 | - |
1 * 40000 + 1 * 35000, endo- and exo-polygalacturonase, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger SA6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75 gel filtration | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.99 | - |
crude enzyme, at pH 4.5 and 40°C | Aspergillus niger |
246 | - |
after 9.1fold purification, at pH 4.5 and 40°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citric pectin + H2O | - |
Aspergillus niger | D-galacturonic acid + ? | - |
? | |
citric pectin + H2O | - |
Aspergillus niger SA6 | D-galacturonic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 40000 + 1 * 35000, endo- and exo-polygalacturonase, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
exo polygalacturonase | - |
Aspergillus niger |
exoPG | - |
Aspergillus niger |
pectinase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the immobilized polygalacturonase is most stable at 50°C and least stable at 70°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | the immobilized polygalacturonase is stable between pH 4.0 and 5.0. The enzyme is however more stable at pH 5.0 | Aspergillus niger |