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Literature summary for 3.2.1.67 extracted from

  • Buga, M.; Ibrahim, S.; Nok, A.
    Physico-chemical characteristics of immobilized polygalacturonase from Aspergillus niger (SA6) (2010), Afr. J. Biotechnol., 9, 8934-8943.
No PubMed abstract available

General Stability

General Stability Organism
calcium alginate-immobilized polygalacturonase exhibits more stability to changes in pH than the temperature. The activity of the immobilized polygalacturonase reduces to 34.56% and 14.81% of the initial activity in the second and third catalytic cycles, respectively Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 40000 + 1 * 35000, endo- and exo-polygalacturonase, SDS-PAGE Aspergillus niger
40000
-
1 * 40000 + 1 * 35000, endo- and exo-polygalacturonase, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger SA6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75 gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.99
-
crude enzyme, at pH 4.5 and 40°C Aspergillus niger
246
-
after 9.1fold purification, at pH 4.5 and 40°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citric pectin + H2O
-
Aspergillus niger D-galacturonic acid + ?
-
?
citric pectin + H2O
-
Aspergillus niger SA6 D-galacturonic acid + ?
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 40000 + 1 * 35000, endo- and exo-polygalacturonase, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
exo polygalacturonase
-
Aspergillus niger
exoPG
-
Aspergillus niger
pectinase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the immobilized polygalacturonase is most stable at 50°C and least stable at 70°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 the immobilized polygalacturonase is stable between pH 4.0 and 5.0. The enzyme is however more stable at pH 5.0 Aspergillus niger