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Literature summary for 3.2.1.67 extracted from
- Purification and characterization of an exo-polygalacturonase from Pycnoporus sanguineus (2009), Mycol. Res., 113, 1404-1410.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Trametes sanguinea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trametes sanguinea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 42000, PGase I, SDS-PAGE and PAGE | Trametes sanguinea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | Trametes sanguinea | - |
galacturonic acid + ? | - |
? |  |
| additional information | Trametes sanguinea | PGase I is an exocellular enzyme releasing galacturonic acid | ? | - |
? | |
| polygalacturonic acid + H2O | Trametes sanguinea | - |
galacturonic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes sanguinea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is N-glycosylated | Trametes sanguinea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble native PGase I 200fold by ammonium sulfate fractionation and gel filtration to homogeneity | Trametes sanguinea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Trametes sanguinea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | - |
Trametes sanguinea | galacturonic acid + ? | - |
? | |
| digalacturonate + H2O | - |
Trametes sanguinea | 2 galacturonate | - |
? | |
| additional information | PGase I is an exocellular enzyme releasing galacturonic acid | Trametes sanguinea | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Trametes sanguinea | galacturonic acid + ? | - |
? | |
| polygalacturonic acid + H2O | preferred substrate | Trametes sanguinea | galacturonic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, PGase I, SDS-PAGE and PAGE | Trametes sanguinea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-PGase | - |
Trametes sanguinea |
| exo-polygalacturonase | - |
Trametes sanguinea |
| PGase I | - |
Trametes sanguinea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | PGase I | Trametes sanguinea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | PGase I | Trametes sanguinea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | at pH 4.8 the activity of the purified PGase I increases from 20 to 60°C, decreasing rapidly beyond | Trametes sanguinea |
| 70 | - |
purified PGase I, 30 min, loss of 57% activity | Trametes sanguinea |
| 80 | - |
purified PGase I, 30 min, loss of 90% activity | Trametes sanguinea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
PGase I | Trametes sanguinea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.8 | 10 | PGase I | Trametes sanguinea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 8 | purified PGase I, stable | Trametes sanguinea |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Trametes sanguinea | growth on citrus fruit pectin induces the enzyme | up |
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Release 2023.1 (January 2023)
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