Any feedback?
Literature summary for 3.2.1.67 extracted from
- Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures (2010), Appl. Biochem. Biotechnol., 160, 1496-1507.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 50% inhibition at 1 mM, complete inhibition at 10 mM | Paecilomyces variotii |  |
| Al3+ | inhibitory at 10 mM | Paecilomyces variotii | |
| Co2+ | inhibitory at 10 mM | Paecilomyces variotii | |
| Fe3+ | inhibitory at 10 mM | Paecilomyces variotii | |
| Hg2+ | inhibitory at 10 mM | Paecilomyces variotii | |
| Mg2+ | inhibitory at 10 mM | Paecilomyces variotii | |
| Mn2+ | inhibitory at 10 mM | Paecilomyces variotii | |
| additional information | no inhibition by Cu2+ and Na+, poor inhibition by K+ | Paecilomyces variotii | |
| Pb2+ | inhibitory at 10 mM | Paecilomyces variotii | |
| Zn2+ | inhibitory at 10 mM | Paecilomyces variotii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Paecilomyces variotii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paecilomyces variotii | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77300 | - |
gel filtration | Paecilomyces variotii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Paecilomyces variotii | the enzyme is highly specific for pectin substrates, overview. It hydrolyzes specifically the terminal glycosidic bond of the polymer and releases galacturonic acid the only degradation product | galacturonic acid + ? | - |
? | |
| polygalacturonic acid + H2O | Paecilomyces variotii | - |
galacturonic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paecilomyces variotii | - |
isolated from decaying hemicellulosic material collected in the state of Sao Paulo, Brazil | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 13fold by anion exchange chromatography and gel filtration to homogeneity | Paecilomyces variotii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 298.5 | - |
purified native enzyme | Paecilomyces variotii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | the enzyme is highly specific for pectin substrates, citric pectins with a degree of esterification of 31%, 63%, and 93% result in 42.7%, 8.3%, and 0.0% relative activity rates, respectively, overview. It hydrolyzes specifically the terminal glycosidic bond of the polymer and releases galacturonic acid the only degradation product | Paecilomyces variotii | galacturonic acid + ? | - |
? | |
| additional information | no activity with xylan, overview | Paecilomyces variotii | ? | - |
? | |
| pectin + H2O | the enzyme is highly specific for pectin substrates, overview. It hydrolyzes specifically the terminal glycosidic bond of the polymer and releases galacturonic acid the only degradation product | Paecilomyces variotii | galacturonic acid + ? | - |
? | |
| polygalacturonic acid + H2O | - |
Paecilomyces variotii | galacturonic acid + ? | - |
? | |
| sodium polypectate + H2O | - |
Paecilomyces variotii | galacturonic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 77300, SDS-PAGE | Paecilomyces variotii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-polygalacturonase | - |
Paecilomyces variotii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Paecilomyces variotii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
purified enzyme, 90 min, completely stable | Paecilomyces variotii |
| 55 | - |
purified enzyme, half-life is 50.6 min | Paecilomyces variotii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Paecilomyces variotii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | purified enzyme, stable | Paecilomyces variotii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Paecilomyces variotii | isoelectric focusing | - |
4.37 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
