Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.67 extracted from

  • Fahmy, A.S.; El-Beih, F.M.; Mohamed, S.A.; Abdel-Gany, S.S.; Abd-Elbaky, E.A.
    Characterization of an exopolygalacturonase from Aspergillus niger (2008), Appl. Biochem. Biotechnol., 149, 205-217.
    View publication on PubMed

Application

Application Comment Organism
food industry properties of PGI may be suitable for food processing Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ at 1 mM 21% inhibition, at 5 mM 35% inhibition Aspergillus niger
Co2+ at 1 mM 19% inhibition, at 5 mM 45% inhibition Aspergillus niger
Cu2+ at 1 mM 27% inhibition, at 5 mM 48% inhibition Aspergillus niger
Hg2+ at 1 mM 17% inhibition, at 5 mM 48% inhibition Aspergillus niger
Mg2+ at 1 mM 8% inhibition, at 5 mM 22% inhibition Aspergillus niger
Mn2+ at 1 mM 57% inhibition, at 5 mM 100% inhibition Aspergillus niger
additional information activity on pectin decreases with an increase in degree of esterification Aspergillus niger
Ni2+ at 1 mM 10% inhibition, at 5 mM 37% inhibition Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger ATCC 9029
-
-
-

Purification (Commentary)

Purification (Comment) Organism
12fold, by anion exchange chromatography and gel filtration, to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.7
-
cell-free broth Aspergillus niger
418.6
-
12fold purified enzyme Aspergillus niger

Storage Stability

Storage Stability Organism
4°C, 9 months Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Aspergillus niger digalacturonate + D-galacturonic acid + ?
-
?
pectin + H2O
-
Aspergillus niger ATCC 9029 digalacturonate + D-galacturonic acid + ?
-
?
polygalacturonic acid + H2O
-
Aspergillus niger ?
-
?
polygalacturonic acid + H2O
-
Aspergillus niger ATCC 9029 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
exopolygalacturonase
-
Aspergillus niger
PGI
-
Aspergillus niger
polygalacturonase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
-
Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8
-
Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing
-
7.6