Application | Comment | Organism |
---|---|---|
food industry | properties of PGI may be suitable for food processing | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 1 mM 21% inhibition, at 5 mM 35% inhibition | Aspergillus niger | |
Co2+ | at 1 mM 19% inhibition, at 5 mM 45% inhibition | Aspergillus niger | |
Cu2+ | at 1 mM 27% inhibition, at 5 mM 48% inhibition | Aspergillus niger | |
Hg2+ | at 1 mM 17% inhibition, at 5 mM 48% inhibition | Aspergillus niger | |
Mg2+ | at 1 mM 8% inhibition, at 5 mM 22% inhibition | Aspergillus niger | |
Mn2+ | at 1 mM 57% inhibition, at 5 mM 100% inhibition | Aspergillus niger | |
additional information | activity on pectin decreases with an increase in degree of esterification | Aspergillus niger | |
Ni2+ | at 1 mM 10% inhibition, at 5 mM 37% inhibition | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger ATCC 9029 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
12fold, by anion exchange chromatography and gel filtration, to homogeneity | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.7 | - |
cell-free broth | Aspergillus niger |
418.6 | - |
12fold purified enzyme | Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C, 9 months | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Aspergillus niger | digalacturonate + D-galacturonic acid + ? | - |
? | |
pectin + H2O | - |
Aspergillus niger ATCC 9029 | digalacturonate + D-galacturonic acid + ? | - |
? | |
polygalacturonic acid + H2O | - |
Aspergillus niger | ? | - |
? | |
polygalacturonic acid + H2O | - |
Aspergillus niger ATCC 9029 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
exopolygalacturonase | - |
Aspergillus niger |
PGI | - |
Aspergillus niger |
polygalacturonase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | - |
Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | isoelectric focusing | - |
7.6 |